ω-agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells

Ricardo Granja; JoséM Fernández-Fernández; Victor Izaguirre; Carmen González-García; Valentin Ceña

doi:10.1016/0014-5793(95)00149-4

ω-agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells

Ricardo Granja, JoséM Fernández-Fernández, Victor Izaguirre, Carmen González-García, Valentin Ceña

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We have studied the contribution of P-type voltage-dependent Ca²⁺ channels to both catacholamine (CA) and ATP secretion from bovine chromaffin cells induced by high K⁺ or nicotine using ω-agatoxin IVA, a selective blocker of P-type voltage-dependent Ca²⁺ channels. We found that high K⁺ (75 mM) induced the release of about 13% of norepinephrine, 5% epinephrine and 11% ATP, and that ω-agatoxin (100 nM) did not affect this secretion. However, both nicotine-induced CA and ATP secretion were significantly blocked (about 50%) by ω-agatoxin IVA (100 nM). In addition, this toxin also reversibly blocked (about 70%) the inward current induced by nicotine in bovine chromaffin cells. The results suggest that, besides its known action of blocking P-type voltage-dependent channels, ω-agatoxin is a potent and reversible blocker of the nicotinic receptor channel in chromaffin cells, and that this action would explain the blockade of nicotine-induced secretion.

Original language	English
Pages (from-to)	15-18
Number of pages	4
Journal	FEBS Letters
Volume	362
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)00149-4
State	Published - 27 Mar 1995

Bibliographical note

Funding Information:
Acknowledgements: This work was supported, in part, by grants from Fundaci6n Salud 2000, Direcci6n General de Investigaci6n Cientifica y T6cnica, Grant PM92-0112, and the European Economic Community, Grant SC1"-CT91-0709. R.G. was supported by a University of Alicante fellowship, J.M.F. by a CAM fellowship, and V.I. by a Gener-alitat Valenciana fellowship.

Keywords

Calcium channel
Chromaffin cell
Nicotinic receptor
P-type
Secretion
ω-Agatoxin IVA

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10.1016/0014-5793(95)00149-4

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title = "ω-agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells",

abstract = "We have studied the contribution of P-type voltage-dependent Ca2+ channels to both catacholamine (CA) and ATP secretion from bovine chromaffin cells induced by high K+ or nicotine using ω-agatoxin IVA, a selective blocker of P-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced the release of about 13% of norepinephrine, 5% epinephrine and 11% ATP, and that ω-agatoxin (100 nM) did not affect this secretion. However, both nicotine-induced CA and ATP secretion were significantly blocked (about 50%) by ω-agatoxin IVA (100 nM). In addition, this toxin also reversibly blocked (about 70%) the inward current induced by nicotine in bovine chromaffin cells. The results suggest that, besides its known action of blocking P-type voltage-dependent channels, ω-agatoxin is a potent and reversible blocker of the nicotinic receptor channel in chromaffin cells, and that this action would explain the blockade of nicotine-induced secretion.",

keywords = "Calcium channel, Chromaffin cell, Nicotinic receptor, P-type, Secretion, ω-Agatoxin IVA",

author = "Ricardo Granja and Jos{\'e}M Fern{\'a}ndez-Fern{\'a}ndez and Victor Izaguirre and Carmen Gonz{\'a}lez-Garc{\'i}a and Valentin Ce{\~n}a",

note = "Funding Information: Acknowledgements: This work was supported, in part, by grants from Fundaci6n Salud 2000, Direcci6n General de Investigaci6n Cientifica y T6cnica, Grant PM92-0112, and the European Economic Community, Grant SC1{"}-CT91-0709. R.G. was supported by a University of Alicante fellowship, J.M.F. by a CAM fellowship, and V.I. by a Gener-alitat Valenciana fellowship.",

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AU - Fernández-Fernández, JoséM

AU - Izaguirre, Victor

AU - González-García, Carmen

AU - Ceña, Valentin

N1 - Funding Information: Acknowledgements: This work was supported, in part, by grants from Fundaci6n Salud 2000, Direcci6n General de Investigaci6n Cientifica y T6cnica, Grant PM92-0112, and the European Economic Community, Grant SC1"-CT91-0709. R.G. was supported by a University of Alicante fellowship, J.M.F. by a CAM fellowship, and V.I. by a Gener-alitat Valenciana fellowship.

PY - 1995/3/27

Y1 - 1995/3/27

N2 - We have studied the contribution of P-type voltage-dependent Ca2+ channels to both catacholamine (CA) and ATP secretion from bovine chromaffin cells induced by high K+ or nicotine using ω-agatoxin IVA, a selective blocker of P-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced the release of about 13% of norepinephrine, 5% epinephrine and 11% ATP, and that ω-agatoxin (100 nM) did not affect this secretion. However, both nicotine-induced CA and ATP secretion were significantly blocked (about 50%) by ω-agatoxin IVA (100 nM). In addition, this toxin also reversibly blocked (about 70%) the inward current induced by nicotine in bovine chromaffin cells. The results suggest that, besides its known action of blocking P-type voltage-dependent channels, ω-agatoxin is a potent and reversible blocker of the nicotinic receptor channel in chromaffin cells, and that this action would explain the blockade of nicotine-induced secretion.

AB - We have studied the contribution of P-type voltage-dependent Ca2+ channels to both catacholamine (CA) and ATP secretion from bovine chromaffin cells induced by high K+ or nicotine using ω-agatoxin IVA, a selective blocker of P-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced the release of about 13% of norepinephrine, 5% epinephrine and 11% ATP, and that ω-agatoxin (100 nM) did not affect this secretion. However, both nicotine-induced CA and ATP secretion were significantly blocked (about 50%) by ω-agatoxin IVA (100 nM). In addition, this toxin also reversibly blocked (about 70%) the inward current induced by nicotine in bovine chromaffin cells. The results suggest that, besides its known action of blocking P-type voltage-dependent channels, ω-agatoxin is a potent and reversible blocker of the nicotinic receptor channel in chromaffin cells, and that this action would explain the blockade of nicotine-induced secretion.

KW - Calcium channel

KW - Chromaffin cell

KW - Nicotinic receptor

KW - P-type

KW - Secretion

KW - ω-Agatoxin IVA

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JO - FEBS Letters

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ER -

ω-agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells

Abstract

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Keywords

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