ω-Conotoxin GVIA blocks nicotine-induced catecholamine secretion by blocking the nicotinic receptor-activated inward currents in bovine chromaffin cells

JoséM M. Fernández; Ricardo Granja; Victor Izaguirre; Carmen González-García; Valentín Ceña

doi:10.1016/0304-3940(95)11558-X

ω-Conotoxin GVIA blocks nicotine-induced catecholamine secretion by blocking the nicotinic receptor-activated inward currents in bovine chromaffin cells

JoséM M. Fernández, Ricardo Granja, Victor Izaguirre, Carmen González-García, Valentín Ceña

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We have studied the contribution of N-type voltage-dependent Ca²⁺ channels to both norepinephrine and epinephrine secretion from bovine chromaffin cells induced by high K⁺ or nicotine using ω-conotoxin GVIA, a selective blocker of N-type voltage-dependent Ca²⁺ channels. We found that high K⁺ (75 mM) induced catecholamine secretion was not affected by exposure of bovine chromaffin cells to ω-conotoxin GVIA (1 μM). However, nicotine-induced both norepinephrine and epinephrine secretion were similarly blocked (about 25%) by ω-conotoxin GVIA (1 μM). This effect could be explained by a potent (about 80%) and reversible blockade of the inward current induced by nicotine receptor activation in bovine chromaffin cells. The results indicate that besides the blockade of N-type voltage-dependent channels, ω-conotoxin GVIA is a potent and reversible blocker of the nicotinic receptor-induced currents in chromaffin cells.

Original language	English
Pages (from-to)	59-62
Number of pages	4
Journal	Neuroscience Letters
Volume	191
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(95)11558-X
State	Published - 19 May 1995

Bibliographical note

Funding Information:
4,8 y---3r---vtinic receptor activation in other neurosecretory systems. -L--This work has been supported, in part, by grants from 15 Fundacion Salud 2000, Direccicin General de Investiga-Fig. 2. Effect of o-conotoxin GVIA on nicotine-induced currents. In- cion Cientifica y Tecnica, grant #PM92-0112 and the (10pM)with an interval of 15 min as described in the text. During theward current induced by three consecutive applications of nicotine European Economic Community, grant #SCl*-CT91-second application of nicotine w-conotoxin GVIA (1 PM) was present, 0709. R.G. is supported by a University of Alicante fel-The figure represent a typical experiment that was repeated four times lowship; J.M.F. by a CAM fellowship and V.I. by a Gen-with similar results. eralitat Valenciana fellowship.

Keywords

Chromaffin cells
Nicotine-induced catecholamine secretion
Nicotinic receptor-induced currents
ω-Conotoxin

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10.1016/0304-3940(95)11558-X

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@article{90c561da918e4bc4ad0afc2daee89253,

title = "ω-Conotoxin GVIA blocks nicotine-induced catecholamine secretion by blocking the nicotinic receptor-activated inward currents in bovine chromaffin cells",

abstract = "We have studied the contribution of N-type voltage-dependent Ca2+ channels to both norepinephrine and epinephrine secretion from bovine chromaffin cells induced by high K+ or nicotine using ω-conotoxin GVIA, a selective blocker of N-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced catecholamine secretion was not affected by exposure of bovine chromaffin cells to ω-conotoxin GVIA (1 μM). However, nicotine-induced both norepinephrine and epinephrine secretion were similarly blocked (about 25%) by ω-conotoxin GVIA (1 μM). This effect could be explained by a potent (about 80%) and reversible blockade of the inward current induced by nicotine receptor activation in bovine chromaffin cells. The results indicate that besides the blockade of N-type voltage-dependent channels, ω-conotoxin GVIA is a potent and reversible blocker of the nicotinic receptor-induced currents in chromaffin cells.",

keywords = "Chromaffin cells, Nicotine-induced catecholamine secretion, Nicotinic receptor-induced currents, ω-Conotoxin",

author = "Fern{\'a}ndez, {Jos{\'e}M M.} and Ricardo Granja and Victor Izaguirre and Carmen Gonz{\'a}lez-Garc{\'i}a and Valent{\'i}n Ce{\~n}a",

note = "Funding Information: 4,8 y---3r---vtinic receptor activation in other neurosecretory systems. -L--This work has been supported, in part, by grants from 15 Fundacion Salud 2000, Direccicin General de Investiga-Fig. 2. Effect of o-conotoxin GVIA on nicotine-induced currents. In- cion Cientifica y Tecnica, grant #PM92-0112 and the (10pM)with an interval of 15 min as described in the text. During theward current induced by three consecutive applications of nicotine European Economic Community, grant #SCl*-CT91-second application of nicotine w-conotoxin GVIA (1 PM) was present, 0709. R.G. is supported by a University of Alicante fel-The figure represent a typical experiment that was repeated four times lowship; J.M.F. by a CAM fellowship and V.I. by a Gen-with similar results. eralitat Valenciana fellowship.",

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T1 - ω-Conotoxin GVIA blocks nicotine-induced catecholamine secretion by blocking the nicotinic receptor-activated inward currents in bovine chromaffin cells

AU - Fernández, JoséM M.

AU - Granja, Ricardo

AU - Izaguirre, Victor

AU - González-García, Carmen

AU - Ceña, Valentín

N1 - Funding Information: 4,8 y---3r---vtinic receptor activation in other neurosecretory systems. -L--This work has been supported, in part, by grants from 15 Fundacion Salud 2000, Direccicin General de Investiga-Fig. 2. Effect of o-conotoxin GVIA on nicotine-induced currents. In- cion Cientifica y Tecnica, grant #PM92-0112 and the (10pM)with an interval of 15 min as described in the text. During theward current induced by three consecutive applications of nicotine European Economic Community, grant #SCl*-CT91-second application of nicotine w-conotoxin GVIA (1 PM) was present, 0709. R.G. is supported by a University of Alicante fel-The figure represent a typical experiment that was repeated four times lowship; J.M.F. by a CAM fellowship and V.I. by a Gen-with similar results. eralitat Valenciana fellowship.

PY - 1995/5/19

Y1 - 1995/5/19

N2 - We have studied the contribution of N-type voltage-dependent Ca2+ channels to both norepinephrine and epinephrine secretion from bovine chromaffin cells induced by high K+ or nicotine using ω-conotoxin GVIA, a selective blocker of N-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced catecholamine secretion was not affected by exposure of bovine chromaffin cells to ω-conotoxin GVIA (1 μM). However, nicotine-induced both norepinephrine and epinephrine secretion were similarly blocked (about 25%) by ω-conotoxin GVIA (1 μM). This effect could be explained by a potent (about 80%) and reversible blockade of the inward current induced by nicotine receptor activation in bovine chromaffin cells. The results indicate that besides the blockade of N-type voltage-dependent channels, ω-conotoxin GVIA is a potent and reversible blocker of the nicotinic receptor-induced currents in chromaffin cells.

AB - We have studied the contribution of N-type voltage-dependent Ca2+ channels to both norepinephrine and epinephrine secretion from bovine chromaffin cells induced by high K+ or nicotine using ω-conotoxin GVIA, a selective blocker of N-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced catecholamine secretion was not affected by exposure of bovine chromaffin cells to ω-conotoxin GVIA (1 μM). However, nicotine-induced both norepinephrine and epinephrine secretion were similarly blocked (about 25%) by ω-conotoxin GVIA (1 μM). This effect could be explained by a potent (about 80%) and reversible blockade of the inward current induced by nicotine receptor activation in bovine chromaffin cells. The results indicate that besides the blockade of N-type voltage-dependent channels, ω-conotoxin GVIA is a potent and reversible blocker of the nicotinic receptor-induced currents in chromaffin cells.

KW - Chromaffin cells

KW - Nicotine-induced catecholamine secretion

KW - Nicotinic receptor-induced currents

KW - ω-Conotoxin

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DO - 10.1016/0304-3940(95)11558-X

M3 - Artículo

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AN - SCOPUS:0028989048

SN - 0304-3940

VL - 191

SP - 59

EP - 62

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

ω-Conotoxin GVIA blocks nicotine-induced catecholamine secretion by blocking the nicotinic receptor-activated inward currents in bovine chromaffin cells

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Keywords

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