Un método para el sexaje por adn de alpaca amplificando el gen SRY mediante PCR

Translated title of the contribution: A method for sexing alpaca DNA by PCR amplification of the SRY gene

C. Nino Arias, L. Wilfredo Huanca

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A method for determining the sex of DNA samples from alpacas has been developed using amplification of the SRY (Sex-determining Region Y chromosome) gene. New oligonucleotide primers were designed according to the SRY gene partial sequence from guanaco (Lama guanicoe). After electrophoresis, a normal male alpaca showed a single SRY band of 146 bp that did not appear in female alpaca samples. After optimization, the PCR procedure for determination of sex was applied to 10 DNA alpaca samples. The sex (genotypic) as diagnosed by PCR corresponded with anatomical sex (phenotypic) in all cases. This study showed that the present method can be applied for sexing DNA samples from alpacas by PCR amplification of the SRY gene.

Translated title of the contributionA method for sexing alpaca DNA by PCR amplification of the SRY gene
Original languageSpanish
Pages (from-to)203-207
Number of pages5
JournalRevista de Investigaciones Veterinarias del Peru
Volume20
Issue number2
StatePublished - 2009

Bibliographical note

Publisher Copyright:
© 2009 Universidad Nacional Mayor de San Marcos. All rights reserved.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Fingerprint Dive into the research topics of 'A method for sexing alpaca DNA by PCR amplification of the SRY gene'. Together they form a unique fingerprint.

Cite this