A Molecular Biologic Study of Enterocytozoon bieneusi in HIV-Infected Patients in Lima, Peru

Irshad M. Sulaiman, Caryn Bern, Robert Gilman, Vitaliano Cama, Vivian Kawai, Daniel Vargas, Eduardo Ticona, Aldo Vivar, Lihua Xiao

Research output: Contribution to conferencePaper

75 Scopus citations

Abstract

A cross-sectional study was conducted to examine the genotype distribution of Enterocytozoon bieneusi in HIV-infected patients who visited two government hospitals in Lima, Peru from January 2000 through March 2003. Microsporidia were detected by microscopy in 105 (3.9%) of 2,672 patients. A total of 212 stool samples from 89 microsporidia-positive patients were genotyped by sequence analysis of the internal transcribed spacer (ITS) region of the rRNA gene. A 392-bp fragment containing the complete ITS region was amplified and sequenced. Multiple alignments and phylogenetic analysis of these ITS sequences identified 11 distinct genotypes of E. bieneusi (Peru-I to Peru-II), 6 of which were new genotypes not reported before. The remaining 5 genotypes had nucleotide sequences identical to those previously reported in humans, cats, pigs, and wild mammals. All the 11 E. bieneusi-genotypes identified are genetically related, and members of the group have been previously found in humans, domestic animals, and some wild mammals. Thus, there is a high genetic diversity of E. bieneusi in humans in Peru, and zoonotic transmission is possible if humans are in close contact with infected animals.
Original languageAmerican English
Pages591-596
Number of pages6
DOIs
StatePublished - 1 Nov 2003
Externally publishedYes
EventJournal of Eukaryotic Microbiology -
Duration: 1 Nov 2003 → …

Conference

ConferenceJournal of Eukaryotic Microbiology
Period1/11/03 → …

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  • Cite this

    Sulaiman, I. M., Bern, C., Gilman, R., Cama, V., Kawai, V., Vargas, D., Ticona, E., Vivar, A., & Xiao, L. (2003). A Molecular Biologic Study of Enterocytozoon bieneusi in HIV-Infected Patients in Lima, Peru. 591-596. Paper presented at Journal of Eukaryotic Microbiology, . https://doi.org/10.1111/j.1550-7408.2003.tb00642.x