A simple method for purification of Strongyloides venezuelensis eggs from rat faeces

W. H. Roldán, F. Martins De Paula, R. C.B. Gryschek

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to develop a simple method to purify Strongyloides eggs from rat faeces using a sucrose gradient centrifugal-flotation technique. This procedure is simple, rapid and possesses a high efficiency in recovering Strongyloides eggs without faecal detritus in less than one hour, thus eliminating the use of complex apparatus and different chemical substances. The possibility of working with pure and live Strongyloides eggs opens up a wide range of future studies on the biology of this parasite. This study constitutes the first report in the scientific literature on purifying Strongyloides eggs using a sucrose density gradient.

Original languageEnglish
Article numbere210
JournalJournal of Helminthology
DOIs
StateAccepted/In press - 2020

Bibliographical note

Publisher Copyright:
Copyright © The Author(s), 2020. Published by Cambridge University Press.

Keywords

  • Strongyloides
  • egg
  • faeces
  • method
  • nematode
  • purification

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