Objective: To evaluate the antitumor activity of the raw extract from biopolymers isolated from the Vibrio sp. marine bacteria in breast cancer induced by N-Methyl-N-nitrosourea (MNU) in rats. Materials and methods: The Vibrio sp. marine bacteria was cultured for seven days, then the raw supernatant was filtered, precipitated and concentrated. MNU was administered in a single dose of 50 mg/kg to 39 Holtzman rats and were daily treated for 9 weeks orally: G1 (n = 13): 0.1 mL/100 g of saline solution; G2 (n = 13): 20 mg/kg of raw extract from Vibrio sp. biopolymers; G3 (n = 13): 100 mg/kg of tamoxifen; G4 (n = 11) received no MNU and only 0.1 mL/100 g of saline solution. Body weight and the appearance of breast tumors identified by palpation were assessed weekly, as well as histopathological examination at the end of treatment. Results: Seventy-seven percent of the rats in the G1 group developed tumors from week 7 onwards in an average of 2.2 tumors per animal; in contrast to the group treated with the raw biopolymer extract and tamoxifen; where only one rat (8%) in each group developed tumors after week nine of induction (p = 0.001). The histopathological results support that all the removed tumors correspond to breast ductal adenocarcinoma with different patterns: solid, papillary and cystic. Likewise, necrotic foci were evidenced in 30% of the tumors of the G1 group. Conclusion: The raw extract of biopolymers isolated from Vibrio sp. present antitumor effect in breast cancer induced in rats.
|Translated title of the contribution||Antitumor activity of Vibrio sp. isolated biopolymers in induced breast cancer in rats|
|Number of pages||7|
|Journal||Revista Peruana de Medicina Experimental y Salud Publica|
|State||Published - 2020|
Bibliographical noteFunding Information:
Agradecimientos: A la Dra. Rita Orozco Moreira del Laboratorio de Microbiología del Instituto del Mar del Perú y al Dr. Hernán Velarde del Instituto de Patología de la Facultad de Medicina de la UNMSM, El proyecto fue financiado por el Vicerrectorado de Investigación de la UNMSM (Resolución Rectoral N.° 01414-R-12. Código del proyecto: 120114011).
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