TY - JOUR
T1 - Aislamiento bacteriológico y caracterización de lesiones histopatológicas en tetra bleeding heart (Hyphessobrycon erythrostigma) de la cuenca amazónica peruana
AU - Adhemir, Valera A.
AU - Jessica, Jurado P.
AU - Alberto, Manchego S.
AU - Nieves, Sandoval C.
N1 - Publisher Copyright:
© 2018 Universidad Nacional Mayor de San Marcos. All Rights Reserved.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2018
Y1 - 2018
N2 - The aim of this study was to determine the presence of bacterial agents and to characterize the histopathological lesions in ornamental tetra bleeding heart (Hyphessobrycon erytrhostigma) fish from a commercial aquarium in the city of Iquitos, Peru. For the microbiological study 60 fish were used, samples were taken from spleen and kidney. Isolation was done on MacConkey agar, Trypticase Soy agar, Pseudomonas Aeromonas selective agar (GSP) and Cytophaga agar and the genus identification was done by Gram staining and biochemical tests. Another 60 fish were used for the histopathological study where the skin, eye, gills, stomach, intestine, liver, spleen, muscle, kidney and peritoneum tissues were analyzed. Seven bacterial genera were isolated: Pseudomonas, Aeromonas, Lactobacillus, Bacillus, Flavobacterium, Staphylocccus and Escherichia. Spores of Mixosporidium sp, hyperplasia and lamella fusion were observed in gills. In the liver and peritoneum, parasitic and bacterial granulomas were found. In muscle, kidney and spleen, bacterial granulomas were found, and in stomach and intestine epithelial cell hyperplasia were found, among other lesions. Results showed that 52.3% (22/42) of the bacterial granulomas were positive for Ziehl Neelsen staining, which showed acid-fast bacilli bacteria. No lesions were found in the eyes.
AB - The aim of this study was to determine the presence of bacterial agents and to characterize the histopathological lesions in ornamental tetra bleeding heart (Hyphessobrycon erytrhostigma) fish from a commercial aquarium in the city of Iquitos, Peru. For the microbiological study 60 fish were used, samples were taken from spleen and kidney. Isolation was done on MacConkey agar, Trypticase Soy agar, Pseudomonas Aeromonas selective agar (GSP) and Cytophaga agar and the genus identification was done by Gram staining and biochemical tests. Another 60 fish were used for the histopathological study where the skin, eye, gills, stomach, intestine, liver, spleen, muscle, kidney and peritoneum tissues were analyzed. Seven bacterial genera were isolated: Pseudomonas, Aeromonas, Lactobacillus, Bacillus, Flavobacterium, Staphylocccus and Escherichia. Spores of Mixosporidium sp, hyperplasia and lamella fusion were observed in gills. In the liver and peritoneum, parasitic and bacterial granulomas were found. In muscle, kidney and spleen, bacterial granulomas were found, and in stomach and intestine epithelial cell hyperplasia were found, among other lesions. Results showed that 52.3% (22/42) of the bacterial granulomas were positive for Ziehl Neelsen staining, which showed acid-fast bacilli bacteria. No lesions were found in the eyes.
KW - Histopathological study
KW - Hyphessobrycon erythrostigma
KW - Microbiological study
UR - http://www.scopus.com/inward/record.url?scp=85045910214&partnerID=8YFLogxK
U2 - 10.15381/rivep.v29i1.14080
DO - 10.15381/rivep.v29i1.14080
M3 - Artículo
AN - SCOPUS:85045910214
SN - 1682-3419
VL - 29
SP - 288
EP - 301
JO - Revista de Investigaciones Veterinarias del Peru
JF - Revista de Investigaciones Veterinarias del Peru
IS - 1
ER -