The aim of this paper is three-fold: (i) to ascertain the phylogenetic position of Sea and Cybdelis in Biblidinae; (ii) to determine the evolutionary relationships among species of Sea and Cybdelis; and (iii) to review the taxonomy of Sea and Cybdelis. The result of the molecular analysis recovers Sea sister to Cybdelis, and this clade sister to Eubagina (sensu Lamas,), rendering Epicaliina (sensu Lamas,) paraphyletic. A new tribe, Cybdelini trib.n. is erected to include Sea and Cybdelis; the other genera formerly included in Epicaliina (sensu Lamas,), Eunica Hübner and Sevenia Koçak are assigned to Eunicini trib.rest., and the remaining genera of Biblidinae to other previously recognized tribes, similar to those implicitly proposed by Wahlberg et al. (). The morphological analysis recovers similar results and the following relationships among species of Cybdelis: C. phaesyla (Hübner) (C. boliviana Salvin (C. mnasylus Doubleday (C. petronita Burmeister stat.rest. + C. thrasylla C. Felder & R. Felder stat.rest.))). Sea and Cybdelis are recognized as distinct genera; a new subspecies, S. sophronia jenkinsi Dias, Siewert & Freitas ssp.n., distributed from Venezuela to Bolivia along the Andes eastern foothills, is described; and C. petronita and C. thrasylla are accepted as valid species. Lectotypes for C. boliviana, C. peruviana Staudinger, C. mnasylus, C. thrasylla, C. petronita and C. mnasylus var. meridionalis Hall and a neotype for Vanessa sophronia Godart are designated. Additionally, dichotomous keys and distribution maps to all taxa are provided; male genitalia and hypandria are illustrated for all species; generic characters of the head, labial palpi and legs are illustrated; immature stages of some species also are figured. The published work has been registered on ZooBank: http://zoobank.org/urn:lsid:zoobank.org:pub:D8EBB649-5E9D-4218-B1F1-7A69DA775FD0.