TY - JOUR
T1 - Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru
AU - Duarte, C. G.
AU - Bonilla, C.
AU - Guimarães, G.
AU - Machado De Avila, R. A.
AU - Mendes, T. M.
AU - Silva, W.
AU - Tintaya, B.
AU - Yarleque, A.
AU - Chávez-Olórtegui, C.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - © 2014 Elsevier Ltd. All rights reserved. In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.
AB - © 2014 Elsevier Ltd. All rights reserved. In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.
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U2 - 10.1016/j.toxicon.2014.10.023
DO - 10.1016/j.toxicon.2014.10.023
M3 - Article
SP - 37
EP - 40
JO - Toxicon
JF - Toxicon
SN - 0041-0101
ER -