© 2014 Elsevier Ltd. All rights reserved. In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.
Duarte, C. G., Bonilla, C., Guimarães, G., Machado De Avila, R. A., Mendes, T. M., Silva, W., Tintaya, B., Yarleque, A., & Chávez-Olórtegui, C. (2015). Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru. Toxicon, 37-40. https://doi.org/10.1016/j.toxicon.2014.10.023