Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

C. G. Duarte; C. Bonilla; G. Guimarães; R. A. Machado De Avila; T. M. Mendes; W. Silva; B. Tintaya; A. Yarleque; C. Chávez-Olórtegui

doi:10.1016/j.toxicon.2014.10.023

Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

C. G. Duarte, C. Bonilla, G. Guimarães, R. A. Machado De Avila, T. M. Mendes, W. Silva, B. Tintaya, A. Yarleque, C. Chávez-Olórtegui

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

© 2014 Elsevier Ltd. All rights reserved. In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.

Original language	American English
Pages (from-to)	37-40
Number of pages	4
Journal	Toxicon
DOIs	https://doi.org/10.1016/j.toxicon.2014.10.023
State	Published - 1 Jan 2015

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Duarte, C. G., Bonilla, C., Guimarães, G., Machado De Avila, R. A., Mendes, T. M., Silva, W., Tintaya, B., Yarleque, A., & Chávez-Olórtegui, C. (2015). Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru. Toxicon, 37-40. https://doi.org/10.1016/j.toxicon.2014.10.023

Duarte, C. G. ; Bonilla, C. ; Guimarães, G. ; Machado De Avila, R. A. ; Mendes, T. M. ; Silva, W. ; Tintaya, B. ; Yarleque, A. ; Chávez-Olórtegui, C. / Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru. In: Toxicon. 2015 ; pp. 37-40.

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abstract = "{\textcopyright} 2014 Elsevier Ltd. All rights reserved. In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.",

author = "Duarte, {C. G.} and C. Bonilla and G. Guimar{\~a}es and {Machado De Avila}, {R. A.} and Mendes, {T. M.} and W. Silva and B. Tintaya and A. Yarleque and C. Ch{\'a}vez-Ol{\'o}rtegui",

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Duarte, CG, Bonilla, C, Guimarães, G, Machado De Avila, RA, Mendes, TM, Silva, W, Tintaya, B, Yarleque, A & Chávez-Olórtegui, C 2015, 'Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru', Toxicon, pp. 37-40. https://doi.org/10.1016/j.toxicon.2014.10.023

Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru. / Duarte, C. G.; Bonilla, C.; Guimarães, G.; Machado De Avila, R. A.; Mendes, T. M.; Silva, W.; Tintaya, B.; Yarleque, A.; Chávez-Olórtegui, C.

In: Toxicon, 01.01.2015, p. 37-40.

Research output: Contribution to journal › Article › peer-review

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AB - © 2014 Elsevier Ltd. All rights reserved. In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.

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