Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

C. G. Duarte; C. Bonilla; G. Guimarães; R. A. Machado De Avila; T. M. Mendes; W. Silva; B. Tintaya; A. Yarleque; C. Chávez-Olórtegui

doi:10.1016/j.toxicon.2014.10.023

Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

C. G. Duarte, C. Bonilla, G. Guimarães, R. A. Machado De Avila, T. M. Mendes, W. Silva, B. Tintaya, A. Yarleque, C. Chávez-Olórtegui

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.

Original language	English
Pages (from-to)	37-40
Number of pages	4
Journal	Toxicon
Volume	93
DOIs	https://doi.org/10.1016/j.toxicon.2014.10.023
State	Published - Jan 2015

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© 2014 Elsevier Ltd. All rights reserved.

Keywords

Antivenom
Immunization
Loxosceles
Neutralization
Recombinant protein

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10.1016/j.toxicon.2014.10.023

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Duarte, C. G., Bonilla, C., Guimarães, G., Machado De Avila, R. A., Mendes, T. M., Silva, W., Tintaya, B., Yarleque, A., & Chávez-Olórtegui, C. (2015). Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru. Toxicon, 93, 37-40. https://doi.org/10.1016/j.toxicon.2014.10.023

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abstract = "In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.",

keywords = "Antivenom, Immunization, Loxosceles, Neutralization, Recombinant protein",

author = "Duarte, {C. G.} and C. Bonilla and G. Guimar{\~a}es and {Machado De Avila}, {R. A.} and Mendes, {T. M.} and W. Silva and B. Tintaya and A. Yarleque and C. Ch{\'a}vez-Ol{\'o}rtegui",

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Duarte, CG, Bonilla, C, Guimarães, G, Machado De Avila, RA, Mendes, TM, Silva, W, Tintaya, B, Yarleque, A & Chávez-Olórtegui, C 2015, 'Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru', Toxicon, vol. 93, pp. 37-40. https://doi.org/10.1016/j.toxicon.2014.10.023

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T1 - Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

AU - Duarte, C. G.

AU - Bonilla, C.

AU - Guimarães, G.

AU - Machado De Avila, R. A.

AU - Mendes, T. M.

AU - Silva, W.

AU - Tintaya, B.

AU - Yarleque, A.

AU - Chávez-Olórtegui, C.

PY - 2015/1

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AB - In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.

KW - Antivenom

KW - Immunization

KW - Loxosceles

KW - Neutralization

KW - Recombinant protein

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DO - 10.1016/j.toxicon.2014.10.023

M3 - Artículo

C2 - 25448393

AN - SCOPUS:84919935913

SN - 0041-0101

VL - 93

SP - 37

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JO - Toxicon

JF - Toxicon

ER -

Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

Abstract

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Keywords

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