Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

C. G. Duarte; C. Bonilla; G. Guimarães; R. A. Machado De Avila; T. M. Mendes; W. Silva; B. Tintaya; A. Yarleque; C. Chávez-Olórtegui

doi:10.1016/j.toxicon.2014.10.023

Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

C. G. Duarte, C. Bonilla, G. Guimarães, R. A. Machado De Avila, T. M. Mendes, W. Silva, B. Tintaya, A. Yarleque, C. Chávez-Olórtegui

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.

Original language	English
Pages (from-to)	37-40
Number of pages	4
Journal	Toxicon
Volume	93
DOIs	https://doi.org/10.1016/j.toxicon.2014.10.023
State	Published - Jan 2015
Externally published	Yes

Bibliographical note

Funding Information:
This research was supported by Instituto Nacional de Salud (INS), Lima, Peru (R.D. N°1099-2009-DG-OGITT-OPE/INS) and executed by specific agreement with the Universidad Nacional Mayor de San Marcos, Lima, Peru. The authors gratefully acknowledge the financial support of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil – CAPES (TOXINOLOGIA N o 23038000825/2011-63 ), Fundação de Amparo a Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG) and by funds of the INCTTOX PROGRAM of Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq) .

Publisher Copyright:
© 2014 Elsevier Ltd. All rights reserved.

Keywords

Antivenom
Immunization
Loxosceles
Neutralization
Recombinant protein

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10.1016/j.toxicon.2014.10.023

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Duarte, C. G., Bonilla, C., Guimarães, G., Machado De Avila, R. A., Mendes, T. M., Silva, W., Tintaya, B., Yarleque, A., & Chávez-Olórtegui, C. (2015). Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru. Toxicon, 93, 37-40. https://doi.org/10.1016/j.toxicon.2014.10.023

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abstract = "In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.",

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author = "Duarte, {C. G.} and C. Bonilla and G. Guimar{\~a}es and {Machado De Avila}, {R. A.} and Mendes, {T. M.} and W. Silva and B. Tintaya and A. Yarleque and C. Ch{\'a}vez-Ol{\'o}rtegui",

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Duarte, CG, Bonilla, C, Guimarães, G, Machado De Avila, RA, Mendes, TM, Silva, W, Tintaya, B, Yarleque, A & Chávez-Olórtegui, C 2015, 'Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru', Toxicon, vol. 93, pp. 37-40. https://doi.org/10.1016/j.toxicon.2014.10.023

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AU - Duarte, C. G.

AU - Bonilla, C.

AU - Guimarães, G.

AU - Machado De Avila, R. A.

AU - Mendes, T. M.

AU - Silva, W.

AU - Tintaya, B.

AU - Yarleque, A.

AU - Chávez-Olórtegui, C.

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AB - In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.

KW - Antivenom

KW - Immunization

KW - Loxosceles

KW - Neutralization

KW - Recombinant protein

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ER -

Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

Abstract

Bibliographical note

Keywords

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