In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.
Bibliographical noteFunding Information:
This research was supported by Instituto Nacional de Salud (INS), Lima, Peru (R.D. N°1099-2009-DG-OGITT-OPE/INS) and executed by specific agreement with the Universidad Nacional Mayor de San Marcos, Lima, Peru. The authors gratefully acknowledge the financial support of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil – CAPES (TOXINOLOGIA N o 23038000825/2011-63 ), Fundação de Amparo a Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG) and by funds of the INCTTOX PROGRAM of Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq) .
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- Recombinant protein