Antibacterial efficacy of calcium hydroxide with iodoform versus calcium hydroxide with camphorated paramonochlorophenol as intrachannel pastes on an Enterococcus faecalis biofilm: A comparative in vitro study

Marisa Jara, Doris Elizabeth Salcedo Moncada, Gerardo Ayala, Romel Watanabe, Daniel Alvítez-Temoche, Frank Mayta-Tovalino

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: The objective of this study was to assess in vitro the antibacterial efficacy of Ca(OH)2with iodoform versus Ca(OH)2with camphorated paramonochlorophenol as intrachannel pastes on an Enterococcus faecalis biofilm. Materials and Methods: The diffusion method was used in wells. The strain used was E. faecalis ATCC 29212. Bile esculin agar was inoculated into 60-well plates of 5 mm in diameter. Three groups were formed: Group 1: Calen PMCC (Ca(OH)2+ camphor paramonochlorophenol); Group 2: Metapex (Ca(OH)2+ iodoform); and Group 3: camphor paramonochlorophenol inoculated with E. faecalis as a positive control. The plates were then incubated at 37°C for 24 h. Bacterial inhibition halos were read. Results: Group 1 presented the highest antimicrobial efficacy with a mean of 16.2 ± 0.6 mm, whereas Group 2 only had an antimicrobial effect of 9.7 ± 1.3 mm. Finally, Group 3 only exposed to the positive control (camphor paramonochlorophenol) showed an effect of 14.6 ± 1.0 mm. The inferential analysis showed statistically significant differences between the antimicrobial effect of the three groups (P = 0.001). Conclusion: Ca(OH)2paste with camphor paramonochlorophenol (Calen PMCC) has a greater antibacterial action on E. faecalis. The iodoform-associated Ca(OH)2paste (Metapex) showed significantly lower antibacterial action against E. faecalis (P 0.05).

Original languageEnglish
Pages (from-to)555-560
Number of pages6
JournalJournal of International Society of Preventive and Community Dentistry
Volume10
Issue number5
DOIs
StatePublished - 1 Sep 2020
Externally publishedYes

Keywords

  • Antibacterial efficacy
  • Enterococcus faecalis
  • in vitro study
  • intraconduction medication

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