Anticonvulsant effect of ethanolic extract of Cyperus articulatus L. leaves on pentylenetetrazol induced seizure in mice

Oscar Herrera-Calderon, Rocio Santiváñez-Acosta, Bertha Pari-Olarte, Edwin Enciso-Roca, Vicente Martin Campos Montes, Jorge Luis Arroyo Acevedo

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24 Scopus citations


Cyperus articulatus (CA) rhizomes have demonstrated different properties on nervous system. However, the leaves still have not studied to treat epilepsy. The aim of this study was to determine the effect of CA ethanolic extract on pentylenetetrazol (PTZ) induced seizures in mice as well as measuring its antioxidant activity in vivo and in vitro. Mice were divided into five groups: (1) control (PTZ 80 mg/kg; i.p.), (2) PTZ-Diazepam (1 mg/kg; i.p.), (3–5) PTZ-CA 50, PTZ-CA 150 and PTZ-CA 300 (50, 150 and 300 mg/kg of CA extract, 30 min prior to each PTZ injection). The PTZ-CA 150 group showed lower seizure scores (P < 0.01), latency (P < 0.01), frequency (P < 0.01) and duration (P < 0.01) than control group. The antioxidant activity of CA extract scavenged DPPH radical showed IC 50 = 16.9 ± 0.1 μg/mL and TEAC = 2.28 ± 0.08, mmol trolox/g of extract, the content of gamma amino butyric acid (GABA) and malondialdehyde (MDA) were significantly high (P < 0.01) at dose of 150 mg/kg (82 ± 1.2 ng/g tissue; 1.0 ± 2.2 mol/g tissue, respectively). The present research demonstrated that CA extract possesses a potential effect to prevent PTZ induced seizures, antioxidant activity in addition to increase GABA levels.

Original languageEnglish
Pages (from-to)95-99
Number of pages5
JournalJournal of Traditional and Complementary Medicine
Issue number1
StatePublished - Jan 2018
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2017 Center for Food and Biomolecules, National Taiwan University


  • Anticonvulsant
  • Antioxidant
  • Cyperus articulatus
  • Pentylenetetrazol


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