Antimicrobial peptides are among the antagonistic metabolites produced by Bifidobacterium against Helicobacter pylori

M. C. Collado, A. González, R. González, M. Hernández, M. A. Ferrús, Y. Sanz

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88 Scopus citations


Sixty acid-resistant Bifidobacterium isolates were recovered from human faeces and identified by genus-specific PCR and RAPD-PCR. Helicobacter pylori strains were isolated from gastric biopsies and identified by species-specific PCR. Twenty-four of the 60 Bifidobacterium isolates were considered to be different strains by RAPD-PCR. Six of the twenty-four different strains were shown to inhibit H. pylori. These antagonistic effects were related to heat-stable proteinaceous compounds, resistant to heating at 100 °C for 10 min, but sensitive to proteases. H. pylori stains showed variable resistance to therapeutic antibiotics (metronidazole and clarithromycin), while all the selected bifidobacteria showed intrinsic resistance to metronidazole. These potentially probiotic bifidobacteria were able to inhibit the growth of both antibiotic sensitive and resistant H. pylori strains. Thus, the synthesis of antimicrobial peptides could be one of the mechanisms of bifidobacteria to combat H. pylori infections.

Original languageEnglish
Pages (from-to)385-391
Number of pages7
JournalInternational Journal of Antimicrobial Agents
Issue number5
StatePublished - May 2005
Externally publishedYes

Bibliographical note

Funding Information:
This work has been supported by Grants GV04B/590 from Consellería de Cultura y Educación de la Generaltitat Valenciana (Spain) and AGL2002-04480-C03-03 from Ministerio de Ciencia y Tecnología (Spain). FPU/MEC (Spain) scholarships to M.C. Collado and A. González are fully acknowledged.


  • Antibiotic resistance
  • Antimicrobial peptides
  • Bacteriocins
  • Bifidobacterium
  • Helicobacter pylori


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