Antimicrobial susceptibility and quinolone resistance mechanism of Arcobacter butzleri isolates from sewage samples in Spain

A. González, R. González, M. A. Ferrús

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

© 2012 by World Scientific Publishing Co. Pte. Ltd. All rights reserved. The direct connection between consumption of Arcobacter contaminated water and human diseases has not been established yet, although it is likely that water may play an important role in the Arcobacter transmission to humans. Fluoroquinolones are potential drugs to treat infections due to these organisms. However, there is evidence of increasing resistance to these antimicrobial agents. This resistance may occur due mutations in a quinolone resistance-determining region (QRDR) of the gyrA gene. In this study, Arcobacter susceptibility to fluoroquinolones was determined by disc diffusion tests and E-test strips. In addition, to elucidate the mechanism of quinolone resistance, we carried out sequence determination and analysis of the QRDR of their gyrA gene. Among the 60 isolates tested, 14 were considered to be resistant to quinolones by disc diffusion tests and E-test strips. The sequencing of the PCR products revealed a mutation in position 254 of the gyrA gene in the 14 resistant A. butzleri isolates. This C-254 to T mutation could be the cause of quinolone resistance as this change was absent in all the susceptible isolates.
Original languageAmerican English
Title of host publicationMicrobes in Applied Research: Current Advances and Challenges, Malaga, Spain, 14 - 16 September 2011
Number of pages5
ISBN (Electronic)9789814405041, 9814405035, 9789814405034
DOIs
StatePublished - 1 Jan 2012
Externally publishedYes

Fingerprint Dive into the research topics of 'Antimicrobial susceptibility and quinolone resistance mechanism of Arcobacter butzleri isolates from sewage samples in Spain'. Together they form a unique fingerprint.

  • Cite this

    González, A., González, R., & Ferrús, M. A. (2012). Antimicrobial susceptibility and quinolone resistance mechanism of Arcobacter butzleri isolates from sewage samples in Spain. In Microbes in Applied Research: Current Advances and Challenges, Malaga, Spain, 14 - 16 September 2011 https://doi.org/10.1142/9789814405041_0100