Antioxidant capacity of chuquiraga spinosa less. "huamanpinta" and prevention of carrageenan-induced inflammation in mice

Hugo Jesús Justil-Guerrero, Jorge Luis Arroyo-Acevedo, Juan Pedro Rojas-Armas, Miriam Palomino-Pacheco, Magaly Villena-Tejada, Wilmer Atilio Segura Vílchez

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To evaluate the antioxidant capacity of Chuquiraga spinosa extracts and prevention of carrageenan-induced inflammation in mice. Methodology: Experimental design: plant species, erythrocytes and male BALB C53 mice, were considered as biological material. Antioxidant capacity was evaluated in 50%, 70%, 96% and aqueous ethanolic extracts by 2,2-Diphenyl-1-Picrylhydrazyl reduction, malondialdehyde inhibition in oxidized erythrocytes with H2O2 and correlating with polyphenol content equivalent to gallic acid/gram dry extract. Inflammation was evaluated by inoculating carrageenan 2% in "subcutaneous air bag"of mice: 1) White, 2) carrageenan, 3) dexamethasone 2 mg/kg, 4-6) ethanolic extract 70% doses 100, 250 and 500 mg/kg respectively; determining nitric oxide, malondialdehyde, total proteins, albumin, leukocytes in exudate and histological changes. Results: Alkaloids, flavonoids, terpenes, phenolic compounds, tannins, carbohydrates, triterpenes, steroids and sesquiterpene lactones were identified; aqueous extract presented greater reduction of 2,2-Diphenyl-1-Picrylhydrazyl (CI50 = 58.99 μg/mL), ethanolic extract 70% presented greater inhibition of malondialdehyde in erythrocytes (CI50 = 16.44 nm/mL); It was observed that the higher the amount of polyphenols, the greater the reduction of 2,2-Diphenyl-1-Picrylhydrazyl (r=-0.909) and the greater the inhibition of malondialdehyde (r=-0.781). With 500 mg/kg of 70% ethanolic extract there was greater anti-inflammatory effect inhibiting malondialdehyde, nitric oxide, albumin, total proteins and leukocytes in 55.55%, 81.92%, 41.20%, 31.51% and 32.45% (p<0.01) respectively and less infiltration of leukocytes and lymphocytes in air sac membrane. Conclusion: The extracts of aerial parts of Chuquiraga spinosa showed antioxidant capacity correlated to polyphenol content. The 70% ethanolic extract prevented inflammation in mice in a dosedependent manner.

Original languageEnglish
Pages (from-to)1287-1296
Number of pages10
JournalPharmacognosy Journal
Volume13
Issue number5
DOIs
StatePublished - Sep 2021
Externally publishedYes

Bibliographical note

Funding Information:
We are grateful for the support of the Research Unit of the Faculty of Medicine of the UNMSM for allowing the execution of the experimental part with animals in the biotherium of the Faculty; we are also grateful to the Direction of the Department of Dynamic Sciences for allowing the execution of the biochemical and phytochemical analysis part of the research in the Pharmacology laboratory.

Publisher Copyright:
© 2021 Phcogj.Com.

Keywords

  • Antioxidant
  • Ethanolic extract
  • Leukocytes
  • Lipoperoxidation
  • Nitric oxide
  • Oxidative stress

Fingerprint

Dive into the research topics of 'Antioxidant capacity of chuquiraga spinosa less. "huamanpinta" and prevention of carrageenan-induced inflammation in mice'. Together they form a unique fingerprint.

Cite this