Asociación entre el fenotipo fisura labiopalatina no sindrómica y marcadores de microsatélite ubicados en 4q

Translated title of the contribution: Association between nonsyndromic cleft lip/palate with microsatellite markers, located in 4q

Mónica Paredes A, Hernán Carreño Z, José Antonio Solá A, Jorge Segú C, Hernán Palomino Z, Rafael Blanco C

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Background: Nonsyndromic cleft lip with or without cleft palate (NSCLP) is a common craniofacial defect. Association studies have suggested that a clefting locus is located on chromosome 4q at or near two microsatellite markers D4S175 and D4S192. Aim: To test the hypothesis on the possible presence of a clefting locus on chromosome 4q. Material and methods: We carried out an association study on a sample of unrelated NSCLP patients, of their unaffected relatives and in controls. Both probands and relatives were further analyzed depending if they originated from simplex or multiplex families. DNA was analyzed with two PCR markers close to the putative NSCLP locus, dinucleotide repeats D4S175 and D4S192. PCR products were resolved by PAGE and visualized by silver staining. Statistical analysis was performed by means of χ2 log ratio. Results: Significant differences between NSCLP and controls were observed when comparing the allele frequency distribution of D4S192 both in the total sample as well as in NSCLP-multiplex and simplex cases. No significant differences for D4S175 were observed in any of the comparisons. Unaffected relatives showed significant differences with controls both for D4S175 and D4S192. Conclusions: Our results support the hypothesis that a NSCLP locus maps on chromosome 4q close to the microsatellite marker D4S192. No differences were observed between NSCLP multiplex and simplex cases versus controls, implying that they do not represent different etiologic entities. The results of the present and previous studies in the same group of patients support the hypothesis that several major interacting genes participate in the etiology of NSCLP.

Translated title of the contributionAssociation between nonsyndromic cleft lip/palate with microsatellite markers, located in 4q
Original languageSpanish
Pages (from-to)1431-1438
Number of pages8
JournalRevista Medica de Chile
Issue number12
StatePublished - Dec 1999
Externally publishedYes


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