Caracterización básica y funcional del semen del Perro sin Pelo del Perúc

Translated title of the contribution: Basic and functional characterization of the Peruvian Hairless Dog semen

Alexei Santiani, María Pérez-Rosales, Carlos Challco

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to characterize the semen of the Peruvian Hairless Dog. Fifteen males, aged 1 to 5 years, registered by the Kennel Club Peruano were used to collect semen (one ejaculated per male) by digital manipulation. A basic spermatogram was performed and macroscopic (volume and colour) and microscopic (sperm motility and concentration, using light field microscopy) characteristics were recorded. Additionally, sperm function tests were performed: sperm viability (using SYBR-14 and propidium iodide), mitochondrial membrane potential (MMP, using MitoTraker Deep Red FM), and acrosomal integrity (using FITC-PSA and propidium iodide) and analysed by flow cytometry with image analysis. The following values were found (mean ± 95% CI): 6.1 ± 2.7 ml volume, opalescent colour; 66.7 ± 10.5% motility; 262.7 ± 20.4 x 106 sperm/ml sperm concentration; 77.7 ± 9.7% of sperm viability; 85.9 ± 5.1% high MMP; 83.6 ± 14.9% acrosomal integrity. These results represent the first report on the seminal characteristics of this breed and could be taken as reference values for a better selection of breeding males and for the diagnosis of reproductive alterations of the breed.

Translated title of the contributionBasic and functional characterization of the Peruvian Hairless Dog semen
Original languageSpanish
Article numbere19251
JournalRevista de Investigaciones Veterinarias del Peru
Volume31
Issue number4
DOIs
StatePublished - 21 Dec 2020

Bibliographical note

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© 2020 Universidad Nacional Mayor de San Marcos. All rights reserved.

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Copyright 2021 Elsevier B.V., All rights reserved.

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