Biochemical and molecular characterization of the hyaluronidase from Bothrops atrox Peruvian snake venom

Dan Erick Vivas Ruiz, Edgar E. Gonzalez-Kozlova, Julio Delgadillo, Pedro M. Palermo, Gustavo Adolfo Sandoval Peña, Fanny Elizabeth Lazo Manrique, Edith Fanincia Rodriguez Quispe, Carlos Chávez-Olórtegui, Armando Yarleque Chocas, Eladio F. Sanchez

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Snake venoms are a rich source of enzymes such as metalloproteinases, serine proteinases phospholipases A2 and myotoxins, that have been well characterized structurally and functionally. However, hyaluronidases (E.C.3.2.1.35) have not been studied extensively. In this study, we describe the biochemical and molecular features of a hyaluronidase (Hyal-Ba) isolated from the venom of the Peruvian snake Bothrops atrox. Hyal-Ba was purified by a combination of ion-exchange and gel filtration chromatography. Purified Hyal-Ba is a 69-kDa (SDS-PAGE) monomeric glycoprotein with an N-terminal amino acid sequence sharing high identity with homologous snake venom hyaluronidases. Detected associated carbohydrates were hexoses (16.38%), hexosamines (2.7%) and sialic acid (0.69%). Hyal-Ba selectively hydrolyzed only hyaluronic acid (HA; specific activity = 437.5 U/mg) but it did not hydrolyze chondroitin sulfate or heparin. The optimal pH and temperature for maximum activity were 6.0 and 40 °C, respectively, and its Km was 0.31 μM. Its activity was inhibited by EDTA, iodoacetate, 2-mercaptoethanol, TLCK and dexamethasone. Na + and K + (0.2 M) positively affect hyaluronidase activity; while Mg 2+ , Br 2+ , Ba 2+ , Cu 2+ , Zn 2+ , and Cd 2+ reduced catalytic activity. Hyal-Ba potentiates the hemorrhagic and hemolytic activity of whole venom, but decreased subplantar edema caused by an L-amino acid oxidase (LAAO). The Hyal-Ba cDNA sequence (2020 bp) encodes 449 amino acid residues, including the catalytic site residues (Glu135, Asp133, Tyr206, Tyr253 and Trp328) and three functional motifs for N-linked glycosylation, which are conserved with other snake hyaluronidases. Spatial modeling of Hyal-Ba displayed a TIM-Barrel (α/β) fold and an EGF-like domain in the C-terminal portion. The phylogenetic analysis of Hyal-Ba with other homologous Hyals showed the monophyly of viperids. Further, Hyal-Ba studies may extend our knowledge of B. atrox toxinology and provides insight to improve the neutralizing strategies of therapeutic antivenoms.

Original languageEnglish
Pages (from-to)33-45
Number of pages13
JournalBiochimie
Volume162
DOIs
StatePublished - Jul 2019
Externally publishedYes

Bibliographical note

Funding Information:
We thank Prof. J.A. Eble from Münster University, Germany for critical reading the manuscript. This work was supported by Vicerrectorado de Investigación y Posgrado – UNMSM (Proyecto N° B17101271 ), Programa Nacional de Innovación para la Competitividad y Productividad - Innovate Perú (Contrato N° 131-FINCyT-IB-2013 ) and Fundação de Amparo à Pesquisa do Estado de Minas Gerais , FAPEMIG , Brazil (Proceso N° HVT-00069-17 ). Part of this report is a dissertation for Master Degree of Julio Delgadillo to Post Graduate School in Molecular Biology, FCB-UNMSM.

Keywords

  • Bothrops atrox pitviper
  • Functional characterization
  • Hyaluronidase
  • Venom

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