Brown marine algae Gongolaria baccata extract protects Caco-2 cells from oxidative stress induced by tert-butyl hydroperoxide

María Aránzazu Martínez, Irma Ares, Marta Martínez, Bernardo Lopez-Torres, Jorge Enrique Maximiliano, Jose Luis Rodríguez, María Rosa Martínez-Larrañaga, Arturo Anadón, Cesar Peteiro, Susana Rubiño, María Hortos

Research output: Contribution to journalArticlepeer-review

Abstract

Gongolaria baccata (S.G. Gmelin) is marine brown seaweed mainly found on the coasts of the Baltic Sea south to the Mediterranean Sea, Canary Islands, Mauritania and Western Sahara. Herein, we report the cell viability and protective effects attributed to molecular mechanisms underlying antioxidant response to survive oxidative stress injuries. Caco-2 cells were submitted to oxidative stress by treatment with tert-butylhydroperoxide (tert-BOOH). The extract prevented cell damage and enhanced activity of antioxidant defenses (NQO1 and GST activities and GSH levels) reduced by treatment with tert-BOOH. The increases of MDA levels, the amount of intracellular ROS and caspase 3/7 activity induced by tert-BOOH were prevented when cells were treated with the G. baccata extract. Moreover, G. baccata extract caused up-regulation of GSTM2, Nrf2, and AKT1 gene expressions, as well as G. baccata extract reduced significantly Bax, BNIP3, APAF1, ERK1, JNK1, MAPK1, P38, P53, NFκB1, TNFα, IL-6, IL-1β and HO-1 gene expressions related to apoptosis, proinflammation and oxidative stress induced by tert-BOOH. These results suggest that G.baccata extract protected the cells against oxidative damage and inflammation; protective effects that could be linked to their bioactive constituents. Hence, this brown seaweed G.baccata extract could be used for the development of functional foods and/or nutraceuticals.

Original languageEnglish
Article number112460
JournalFood and Chemical Toxicology
Volume156
DOIs
StatePublished - Oct 2021

Bibliographical note

Funding Information:
This work was supported by the Project Ref. RTA2015-00010-C03 from the Ministerio de Economía, Industria y Competitividad, Spain , and Project Ref. PID 2020-15979RR-C33 from the Ministerio de Ciencia e Innovación, Spain .

Funding Information:
This work was supported by the Project Ref. RTA2015-00010-C03 from the Ministerio de Econom?a, Industria y Competitividad, Spain, and Project Ref. PID 2020-15979RR-C33 from the Ministerio de Ciencia e Innovaci?n, Spain.

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

  • Caco-2 cells
  • G. baccata extract
  • Oxidative stress
  • Phenolic and carbohydrate derivatives

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