TY - JOUR
T1 - Caracterización de metalo-β-Lactamasas en aislados clínicos de pseudomonas aeruginosa recuperados de pacientes hospitalizados en el hospital militar central
AU - Salvador-Luján, Gina
AU - Garcia De La Guarda, Ruth Hortensia
AU - Gonzales-Escalante, Edgar
N1 - Publisher Copyright:
© 2018, Instituto Nacional de Salud. All rights reserved.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The bla IMP gene was found in 23/24 (95.8%) and bla VIM in 1/24 (4.2%). No isolate had bla NDM . The bla IMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.
AB - Hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The bla IMP gene was found in 23/24 (95.8%) and bla VIM in 1/24 (4.2%). No isolate had bla NDM . The bla IMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.
KW - Beta-lactam resistance
KW - Beta-lactamases
KW - Carbapenems
KW - Peru
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=85061147022&partnerID=8YFLogxK
U2 - 10.17843/rpmesp.2018.354.3755
DO - 10.17843/rpmesp.2018.354.3755
M3 - Artículo
C2 - 30726418
AN - SCOPUS:85061147022
SN - 1726-4634
VL - 35
SP - 363
EP - 641
JO - Revista Peruana de Medicina de Experimental y Salud Publica
JF - Revista Peruana de Medicina de Experimental y Salud Publica
IS - 4
ER -
