Caracterización de metalo-β-Lactamasas en aislados clínicos de pseudomonas aeruginosa recuperados de pacientes hospitalizados en el hospital militar central

Gina Salvador-Luján; Ruth García-de-la-guarda; Edgar Gonzales-Escalante

doi:10.17843/rpmesp.2018.354.3755

Caracterización de metalo-β-Lactamasas en aislados clínicos de pseudomonas aeruginosa recuperados de pacientes hospitalizados en el hospital militar central

Translated title of the contribution: Characterization of metallo-β-Lactamase in clinical isolates of pseudomonas aeruginosa retrieved from patients hospitalized in the central military hospital

Gina Salvador-Luján, Ruth García-de-la-guarda, Edgar Gonzales-Escalante

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The bla _IMP gene was found in 23/24 (95.8%) and bla _VIM in 1/24 (4.2%). No isolate had bla ^NDM . The bla _IMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.

Translated title of the contribution	Characterization of metallo-β-Lactamase in clinical isolates of pseudomonas aeruginosa retrieved from patients hospitalized in the central military hospital
Original language	Spanish
Pages (from-to)	363-641
Number of pages	279
Journal	Revista Peruana de Medicina Experimental y Salud Publica
Volume	35
Issue number	4
DOIs	https://doi.org/10.17843/rpmesp.2018.354.3755
State	Published - 1 Oct 2018
Externally published	Yes

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© 2018, Instituto Nacional de Salud. All rights reserved.

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10.17843/rpmesp.2018.354.3755

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title = "Caracterizaci{\'o}n de metalo-β-Lactamasas en aislados cl{\'i}nicos de pseudomonas aeruginosa recuperados de pacientes hospitalizados en el hospital militar central",

abstract = " Hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The bla IMP gene was found in 23/24 (95.8%) and bla VIM in 1/24 (4.2%). No isolate had bla NDM . The bla IMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.",

keywords = "Beta-lactam resistance, Beta-lactamases, Carbapenems, Peru, Pseudomonas aeruginosa",

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Caracterización de metalo-β-Lactamasas en aislados clínicos de pseudomonas aeruginosa recuperados de pacientes hospitalizados en el hospital militar central. / Salvador-Luján, Gina; García-de-la-guarda, Ruth; Gonzales-Escalante, Edgar.
In: Revista Peruana de Medicina Experimental y Salud Publica, Vol. 35, No. 4, 01.10.2018, p. 363-641.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Caracterización de metalo-β-Lactamasas en aislados clínicos de pseudomonas aeruginosa recuperados de pacientes hospitalizados en el hospital militar central

AU - Salvador-Luján, Gina

AU - García-de-la-guarda, Ruth

AU - Gonzales-Escalante, Edgar

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The bla IMP gene was found in 23/24 (95.8%) and bla VIM in 1/24 (4.2%). No isolate had bla NDM . The bla IMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.

AB - Hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The bla IMP gene was found in 23/24 (95.8%) and bla VIM in 1/24 (4.2%). No isolate had bla NDM . The bla IMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.

KW - Beta-lactam resistance

KW - Beta-lactamases

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KW - Peru

KW - Pseudomonas aeruginosa

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Caracterización de metalo-β-Lactamasas en aislados clínicos de pseudomonas aeruginosa recuperados de pacientes hospitalizados en el hospital militar central

Abstract

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