Characterization of 16 microsatellite markers for the Oreinotinus Clade of Viburnum (Adoxaceae)

Syndi Barish, Mónica Arakaki, Erika J. Edwards, Michael J. Donoghue, Wendy L. Clement

Research output: Contribution to journalArticlepeer-review

Abstract

Published by the Botanical Society of America. Premise of the study: Microsatellite loci were isolated from four species of Viburnum (Adoxaceae) to study population structure and assess species boundaries among morphologically similar South American Viburnum species of the Oreinotinus clade. Methods and Results: Using a microsatellite-enriched library and mining next-generation sequence data, 16 microsatellites were developed. Each locus was tested on two populations of V. triphyllum and one population of V. pichinchense. For nuclear loci, one to 13 alleles were recovered, expected heterozygosity ranged from 0 to 0.8975, Simpson diversity index ranged from 0.0167 to 1.000, and Shannon diversity index ranged from 0 to 2.3670 in a given population. For the mitochondrial locus, three to six alleles were recovered and unbiased haploid diversity values ranged from 0.756 to 0.853 in a given population. Conclusions: The 16 microsatellite loci developed for the Oreinotinus clade (Viburnum, Adoxaceae) will inform investigations of population structure and species boundaries within this group.
Original languageAmerican English
JournalApplications in Plant Sciences
Volume4
Issue number12
DOIs
StatePublished - 1 Dec 2016

Keywords

  • Adoxaceae
  • Viburnum dentatum
  • Viburnum hallii
  • Viburnum pichinchense
  • Viburnum trilobum
  • Viburnum triphyllum
  • genetic diversity

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