Caracterización de actinomicetos de sedimento marino y su actividad antagonista frente a Vibrio sp. aislados de «langostino blanco» Litopenaeus vannamei (Boone, 1931)

Translated title of the contribution: Characterization of actinomycetes of marine sediment and their antagonistic activity against vibrio sp. Isolated from «whiteleg shrimp» litopenaeus vannamei (Boone, 1931)

J. Ulrike Tarazona, Jorge Leon Quispe, C. Nadia Galindo, Marisol Vallejo, Emilio Marguet

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The aim of the present study was to evaluate the antagonistic activity of a collection of 24 actinomycetes of marine sediment against wild species of Vibrio. Actinomycetes were characterized by their growth in Marine Agar and their extracellular enzymatic activity on different substrates. The antagonist activity was determined by in vitro tests against six strains of Vibrio isolated from Litopenaeus vannamei. Actinomycetes with greater antagonism were evaluated by their growth in standard ISP media and observations of microphotographs by scanning electron microscopy (SEM) techniques were done. The Vibrio strains were evaluated and identified by conventional biochemical techniques and confirmed by PCR. The results indicated that all actinomycetes showed at least some extracellular multi-enzymatic activity, predominating those that hydrolyze starch (79%). Antagonism tests indicated that 54% (13/24) of actinomycetes had anti-Vibrio activity, being the strains M10-77 and M11-116 (B) those that produced the largest inhibition halos. On the other hand, the sequence-based identification of the 16S rRNA gene corroborated the relationship of the isolated strains of L. vannamei with the genus Vibrio; likewise, some of these actinobacteria were identified as Streptomyces.

Translated title of the contributionCharacterization of actinomycetes of marine sediment and their antagonistic activity against vibrio sp. Isolated from «whiteleg shrimp» litopenaeus vannamei (Boone, 1931)
Original languageSpanish
Pages (from-to)676-691
Number of pages16
JournalRevista de Investigaciones Veterinarias del Peru
Volume29
Issue number2
DOIs
StatePublished - 2018
Externally publishedYes

Fingerprint

Dive into the research topics of 'Characterization of actinomycetes of marine sediment and their antagonistic activity against vibrio sp. Isolated from «whiteleg shrimp» litopenaeus vannamei (Boone, 1931)'. Together they form a unique fingerprint.

Cite this