TY - JOUR
T1 - Characterization of metallo-β-Lactamase in clinical isolates of pseudomonas aeruginosa retrieved from patients hospitalized in the central military hospital
AU - Salvador-Luján, Gina
AU - García-de-la-guarda, Ruth
AU - Gonzales-Escalante, Edgar
PY - 2018/10/1
Y1 - 2018/10/1
N2 - © 2018, Instituto Nacional de Salud. All rights reserved. Hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The bla IMP gene was found in 23/24 (95.8%) and bla VIM in 1/24 (4.2%). No isolate had bla NDM . The bla IMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.
AB - © 2018, Instituto Nacional de Salud. All rights reserved. Hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The bla IMP gene was found in 23/24 (95.8%) and bla VIM in 1/24 (4.2%). No isolate had bla NDM . The bla IMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.
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U2 - 10.17843/rpmesp.2018.354.3755
DO - 10.17843/rpmesp.2018.354.3755
M3 - Article
SN - 1726-4634
SP - 363
EP - 641
JO - Revista Peruana de Medicina Experimental y Salud Publica
JF - Revista Peruana de Medicina Experimental y Salud Publica
ER -
