Characterization of metallo-β-Lactamase in clinical isolates of pseudomonas aeruginosa retrieved from patients hospitalized in the central military hospital

Gina Salvador-Luján, Ruth García-de-la-guarda, Edgar Gonzales-Escalante

Research output: Contribution to journalArticle

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Abstract

© 2018, Instituto Nacional de Salud. All rights reserved. Hat codify metallo-β-lactamases (MBL) in clinical isolates of Pseudomona aeruginosa (P. aeruginosa), a cross-sectional study was conducted in the Central Military Hospital (HMC) of Lima, Peru, between January and September, 2016. Seventy-six (76) isolates of P. aeruginosa with diminished sensitivity to carbapenemes and resistant to ceftazidime were analyzed. The phenotype detection of MBL was made by double disc synergy between imipenem and meropenem versus ethylenediaminetetraacetic acid (EDTA), and the identification of the genes was performed by polymerase chain reaction. Of the 76 isolates, 24 (31.6%) were positive for MBL by the phenotype method, genetically confirming all. The bla IMP gene was found in 23/24 (95.8%) and bla VIM in 1/24 (4.2%). No isolate had bla NDM . The bla IMP gene turned out to be the most frequent among clinical isolates of P. aeruginosa not sensitive to carbapenemics at this Hospital.
Original languageAmerican English
Pages (from-to)363-641
Number of pages279
JournalRevista Peruana de Medicina Experimental y Salud Publica
DOIs
StatePublished - 1 Oct 2018

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