© 2019 Universidad Nacional Mayor de San Marcos. All rights reserved. The objective of the study was to characterize the biosecurity measures of the poultry farms located in the province of Coronel Portillo, Ucayali - Peru. Fifty-three farms distributed in the districts of Campo Verde, Yarinacocha, Manantay, Callería and Nueva Requena were evaluated through a survey to the manager of the establishment. The data collected was verified visually and through records. Biosecurity measures were evaluated according to compliance, being 1 = met and 0 = not met. Twenty-nine farms were in a sanitary void, so the data of uniformity of the flocks and the handling of feed were not obtained. Within the seven obligatory biosecurity measures, all the farms had a disinfection program and 98.1% had a disposal system for dead birds; nevertheless, no farm complied with the seven measures, being the biggest problem the lack of human capacity building, where only 15.1% of farms complied. The complementary biosecurity measures were implemented in a variable way between the farms and between the districts.