Concordance between indirect hemagglutination and indirect immunofluorescence tests to determine the prevalence of Toxoplasma gondii in sheep

O. Sandra Huerta, V. Amanda Chávez, A. Eva Casas, P. Néstor Falcón, T. Fernando Raymundo

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

© 2006 Universidad Nacional Mayor de San Marcos. All rights reserved. The objective of this study was to measure the degree of concordance between the Indirect hemaglutination (HAI) and Indirect immunofluorescence (IFI) tests for Toxoplasma gondii detection in sheep. A total of 310 sera were collected from ewes of different ages at Fundo Santa María, district of Nuñoa, province of Melgar, Puno. The Kappa (K) value was 24%, showing that the degree of association between both techniques was of a regular type. The Mc Nemar test showed significative differences between both tests, so one cannot replace the other. The seroprevalence for T. gondii was 50.3 ± 5.6% through HAI test and 88.1 ± 3.5% through IFI test. Seroprevalence in animals <1, 1-2, 2-3, and >3 years of age was 23.2, 33.3, 52.1, and 58.7% through HAI test and 72.0, 90.0, 91.2, and 90.5% respectively through IFI test.
Original languageAmerican English
Pages (from-to)178-183
Number of pages6
JournalRevista de Investigaciones Veterinarias del Peru
StatePublished - 1 Jan 2006

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