Infecciones concurrentes por dos serotipos del virus dengue durante un brote en el noroeste de perú, 2008

Translated title of the contribution: Concurrent infections by two dengue virus serotypes during an outbreak in northwestern peru, 2008

Enrique Mamani, Dana Figueroa, María Paquita García, María del Carmen Garaycochea, Edwar J. Pozo

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Objetives. To establish the existence of concurrent infections by different dengue virus (DENV) serotypes in an outbreak in the Northwestern in Peru during 2008. Material and methods. 73 serum samples from patients with dengue were analyzed during an outbreak that occurred in Northwestern in Peru between May and June 2008. Molecular biology techniques were used to serotype the DENV, thus, firstly the viral RNA viral was extracted using Viral QIAamp RNA mini kit (Qiagen, Valencia, California, USA), then the viral cDNA fragments were reverse transcripted and amplified by means of the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and the RT-Nested PCR region techniques and finally, genetic sequencing of the viral cDNA fragments were performed using the Big Dye Terminator v.3,1 kit. Results. The 73 dengue cases presented infections by different serotypes: 34 (46.6%) by DENV-3, 29 (39.7%) by DENV-1, 4 (5.5%) by DENV-4, and 6 (8.2%) concurrent infections by DENV-1 and DENV-3. The most frequent clinical manifestations observed among dengue patients were fever and headache (100%), myalgia (94.5%), ocular pain (83.6%), arthralgia (78.1%), shivers (63.0%), nausea/vomiting (38.4%), positive tourniquet test (30.1%), and rash (20.5%). All patients with concurrent infections presented light clinical course of dengue fever (Df) except one patient who had moderate hemorrhagic manifestations. Conclusion. This is the first Peruvian report of patients with concurrent infections of two DENV serotypes without severe clinical manifestations.

Translated title of the contributionConcurrent infections by two dengue virus serotypes during an outbreak in northwestern peru, 2008
Original languageSpanish
Pages (from-to)16-21
Number of pages6
JournalRevista Peruana de Medicina de Experimental y Salud Publica
Volume27
Issue number1
DOIs
StatePublished - 2010

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