TY - JOUR
T1 - Culture isolation and molecular identification of blastocystis sp. In Brazilian human isolates
T2 - Preliminary results
AU - de Melo, Gessica Baptista
AU - Roldan, William
AU - Malta, Fernanda de Mello
AU - Lescano, Susana Angelica Zevallos
AU - Castilho, Vera Lúcia
AU - Gonçalves, Elenice Messias Do Nascimento
AU - de Paula, Fabiana Martins
AU - Gryschek, Ronaldo Cesar Borges
N1 - Publisher Copyright:
© 2020, Instituto de Medicina Tropical de Sao Paulo. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Blastocystis sp. is a protist commonly found in stool samples of humans and animals. Biological and genetic factors of this organism remain controversial. The present study aimed to develop and implement the Blastocystis in vitro culture of Brazilian human isolates for routine use. The fecal isolates (n = 20) were maintained in our laboratory by several passages in Pavlova’s medium. Cultures were monitored every 72 h by light microscopy. Genomic DNA was extracted to identify the subtypes (STs). In most isolates, the vacuolar form was prevalent. The amoeboid, granular and cystic forms were observed during in vitro cultivation. STs 1, 2, 3, 4 and 7 were identified. Our preliminary results show the generation time and forms present in the in vitro culture of Blastocystis subtypes isolated from Brazilian human isolates. Therefore, we emphasize the use of in vitro culture as a tool in future studies for the better understanding of the biological aspects of Blastocystis sp.
AB - Blastocystis sp. is a protist commonly found in stool samples of humans and animals. Biological and genetic factors of this organism remain controversial. The present study aimed to develop and implement the Blastocystis in vitro culture of Brazilian human isolates for routine use. The fecal isolates (n = 20) were maintained in our laboratory by several passages in Pavlova’s medium. Cultures were monitored every 72 h by light microscopy. Genomic DNA was extracted to identify the subtypes (STs). In most isolates, the vacuolar form was prevalent. The amoeboid, granular and cystic forms were observed during in vitro cultivation. STs 1, 2, 3, 4 and 7 were identified. Our preliminary results show the generation time and forms present in the in vitro culture of Blastocystis subtypes isolated from Brazilian human isolates. Therefore, we emphasize the use of in vitro culture as a tool in future studies for the better understanding of the biological aspects of Blastocystis sp.
KW - Blastocystis sp
KW - Brazil
KW - In vitro culture
KW - Subtypes
UR - http://www.scopus.com/inward/record.url?scp=85088270442&partnerID=8YFLogxK
U2 - 10.1590/S1678-9946202062051
DO - 10.1590/S1678-9946202062051
M3 - Artículo
C2 - 32667389
AN - SCOPUS:85088270442
SN - 0036-4665
VL - 62
SP - 1
EP - 5
JO - Revista do Instituto de Medicina Tropical de Sao Paulo
JF - Revista do Instituto de Medicina Tropical de Sao Paulo
M1 - e51
ER -