Blastocystis sp. is a protist commonly found in stool samples of humans and animals. Biological and genetic factors of this organism remain controversial. The present study aimed to develop and implement the Blastocystis in vitro culture of Brazilian human isolates for routine use. The fecal isolates (n = 20) were maintained in our laboratory by several passages in Pavlova’s medium. Cultures were monitored every 72 h by light microscopy. Genomic DNA was extracted to identify the subtypes (STs). In most isolates, the vacuolar form was prevalent. The amoeboid, granular and cystic forms were observed during in vitro cultivation. STs 1, 2, 3, 4 and 7 were identified. Our preliminary results show the generation time and forms present in the in vitro culture of Blastocystis subtypes isolated from Brazilian human isolates. Therefore, we emphasize the use of in vitro culture as a tool in future studies for the better understanding of the biological aspects of Blastocystis sp.
|Number of pages||5|
|Journal||Revista do Instituto de Medicina Tropical de Sao Paulo|
|State||Published - 2020|
Bibliographical noteFunding Information:
This study was supported by grants from the Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (FAPESP grant N? 2015/18213-6) and Coordena??o de Aperfei?oamento de Pessoa de N?vel Superior (CAPES grant N? 1749729).
This study was supported by grants from the Fundação de
Amparo à Pesquisa do Estado de São Paulo (FAPESP grant Nº 2015/18213-6) and Coordenação de Aperfeiçoamento de Pessoa de Nível Superior (CAPES grant Nº 1749729).
© 2020, Instituto de Medicina Tropical de Sao Paulo. All rights reserved.
- Blastocystis sp
- In vitro culture