Culture isolation and molecular identification of blastocystis sp. In Brazilian human isolates: Preliminary results

Gessica Baptista de Melo, William Roldan, Fernanda de Mello Malta, Susana Angelica Zevallos Lescano, Vera Lúcia Castilho, Elenice Messias Do Nascimento Gonçalves, Fabiana Martins de Paula, Ronaldo Cesar Borges Gryschek

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Blastocystis sp. is a protist commonly found in stool samples of humans and animals. Biological and genetic factors of this organism remain controversial. The present study aimed to develop and implement the Blastocystis in vitro culture of Brazilian human isolates for routine use. The fecal isolates (n = 20) were maintained in our laboratory by several passages in Pavlova’s medium. Cultures were monitored every 72 h by light microscopy. Genomic DNA was extracted to identify the subtypes (STs). In most isolates, the vacuolar form was prevalent. The amoeboid, granular and cystic forms were observed during in vitro cultivation. STs 1, 2, 3, 4 and 7 were identified. Our preliminary results show the generation time and forms present in the in vitro culture of Blastocystis subtypes isolated from Brazilian human isolates. Therefore, we emphasize the use of in vitro culture as a tool in future studies for the better understanding of the biological aspects of Blastocystis sp.

Original languageEnglish
Article numbere51
Pages (from-to)1-5
Number of pages5
JournalRevista do Instituto de Medicina Tropical de Sao Paulo
Volume62
DOIs
StatePublished - 2020

Bibliographical note

Funding Information:
This study was supported by grants from the Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (FAPESP grant N? 2015/18213-6) and Coordena??o de Aperfei?oamento de Pessoa de N?vel Superior (CAPES grant N? 1749729).

Funding Information:
This study was supported by grants from the Fundação de

Funding Information:
Amparo à Pesquisa do Estado de São Paulo (FAPESP grant Nº 2015/18213-6) and Coordenação de Aperfeiçoamento de Pessoa de Nível Superior (CAPES grant Nº 1749729).

Publisher Copyright:
© 2020, Instituto de Medicina Tropical de Sao Paulo. All rights reserved.

Keywords

  • Blastocystis sp
  • Brazil
  • In vitro culture
  • Subtypes

Fingerprint Dive into the research topics of 'Culture isolation and molecular identification of blastocystis sp. In Brazilian human isolates: Preliminary results'. Together they form a unique fingerprint.

Cite this