Detección fenotípica de mecanismos de resistencia antimicrobiana de Escherichia coli aisladas de infecciones entéricas de porcinos provenientes de granjas de producción tecnificada

C. Michelle Monterroso; R. Guillermo Salvatierra; S. André Sedano; E. Sonia Calle

doi:10.15381/rivep.v30i1.15670

Detección fenotípica de mecanismos de resistencia antimicrobiana de Escherichia coli aisladas de infecciones entéricas de porcinos provenientes de granjas de producción tecnificada

Translated title of the contribution: Phenotypic detection of antimicrobial resistance mechanisms of Escherichia coli isolates from enteric infections in pigs from technified farms

C. Michelle Monterroso, R. Guillermo Salvatierra, S. André Sedano, E. Sonia Calle

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The aim of this study was to detect the antimicrobial resistance mechanisms of 36 Escherichia coli isolates to beta-lactams, quinolones and aminoglycosides using the Kirby-Bauer technique. Thirty-six E. coli isolates from pigs of technified production farms obtained during the 2010-2015 period were used. Fifteen antimicrobials of importance in human and veterinary medicine were tested. The isolates showed resistance mainly to nalidixic acid (89%, 32/36), cloxacillin (83%, 30/36) and amoxicillin-clavulanic acid (69%, 25/36). Only 3% (1/36) were AmpC producers, 42% (15/36) showed a possible mutation in gyrA and 14% (5/36) at least two possible mutations in gyrA or gyrA+parC. In addition, 33% (12/36) showed high probabilities of presence of qnr genes. The enzymes associated to aminoglycoside resistance mechanism were positive in 39% (14/36) to AAC (6’), 28% (10/36) to ANT (2") and 11% (4/36) to AAC (3) IV.

Translated title of the contribution	Phenotypic detection of antimicrobial resistance mechanisms of Escherichia coli isolates from enteric infections in pigs from technified farms
Original language	Spanish
Pages (from-to)	455-464
Number of pages	10
Journal	Revista de Investigaciones Veterinarias del Peru
Volume	30
Issue number	1
DOIs	https://doi.org/10.15381/rivep.v30i1.15670
State	Published - 2019
Externally published	Yes

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10.15381/rivep.v30i1.15670

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Michelle Monterroso, C., Guillermo Salvatierra, R., André Sedano, S., & Sonia Calle, E. (2019). Detección fenotípica de mecanismos de resistencia antimicrobiana de Escherichia coli aisladas de infecciones entéricas de porcinos provenientes de granjas de producción tecnificada. Revista de Investigaciones Veterinarias del Peru, 30(1), 455-464. https://doi.org/10.15381/rivep.v30i1.15670

@article{bce95cbb313f442d9b6a1de3149cdb50,

title = "Detecci{\'o}n fenot{\'i}pica de mecanismos de resistencia antimicrobiana de Escherichia coli aisladas de infecciones ent{\'e}ricas de porcinos provenientes de granjas de producci{\'o}n tecnificada",

abstract = "The aim of this study was to detect the antimicrobial resistance mechanisms of 36 Escherichia coli isolates to beta-lactams, quinolones and aminoglycosides using the Kirby-Bauer technique. Thirty-six E. coli isolates from pigs of technified production farms obtained during the 2010-2015 period were used. Fifteen antimicrobials of importance in human and veterinary medicine were tested. The isolates showed resistance mainly to nalidixic acid (89%, 32/36), cloxacillin (83%, 30/36) and amoxicillin-clavulanic acid (69%, 25/36). Only 3% (1/36) were AmpC producers, 42% (15/36) showed a possible mutation in gyrA and 14% (5/36) at least two possible mutations in gyrA or gyrA+parC. In addition, 33% (12/36) showed high probabilities of presence of qnr genes. The enzymes associated to aminoglycoside resistance mechanism were positive in 39% (14/36) to AAC (6{\textquoteright}), 28% (10/36) to ANT (2{"}) and 11% (4/36) to AAC (3) IV.",

keywords = "Antimicrobial resistance, Escherichia coli, Pigs, Public health, Resistance mechanisms",

author = "{Michelle Monterroso}, C. and {Guillermo Salvatierra}, R. and {Andr{\'e} Sedano}, S. and {Sonia Calle}, E.",

year = "2019",

doi = "10.15381/rivep.v30i1.15670",

language = "Espa{\~n}ol",

volume = "30",

pages = "455--464",

journal = "Revista de Investigaciones Veterinarias del Peru",

issn = "1682-3419",

publisher = "Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos",

number = "1",

}

Michelle Monterroso, C, Guillermo Salvatierra, R, André Sedano, S & Sonia Calle, E 2019, 'Detección fenotípica de mecanismos de resistencia antimicrobiana de Escherichia coli aisladas de infecciones entéricas de porcinos provenientes de granjas de producción tecnificada', Revista de Investigaciones Veterinarias del Peru, vol. 30, no. 1, pp. 455-464. https://doi.org/10.15381/rivep.v30i1.15670

Detección fenotípica de mecanismos de resistencia antimicrobiana de Escherichia coli aisladas de infecciones entéricas de porcinos provenientes de granjas de producción tecnificada. / Michelle Monterroso, C.; Guillermo Salvatierra, R.; André Sedano, S. et al.

In: Revista de Investigaciones Veterinarias del Peru, Vol. 30, No. 1, 2019, p. 455-464.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Detección fenotípica de mecanismos de resistencia antimicrobiana de Escherichia coli aisladas de infecciones entéricas de porcinos provenientes de granjas de producción tecnificada

AU - Michelle Monterroso, C.

AU - Guillermo Salvatierra, R.

AU - André Sedano, S.

AU - Sonia Calle, E.

PY - 2019

Y1 - 2019

N2 - The aim of this study was to detect the antimicrobial resistance mechanisms of 36 Escherichia coli isolates to beta-lactams, quinolones and aminoglycosides using the Kirby-Bauer technique. Thirty-six E. coli isolates from pigs of technified production farms obtained during the 2010-2015 period were used. Fifteen antimicrobials of importance in human and veterinary medicine were tested. The isolates showed resistance mainly to nalidixic acid (89%, 32/36), cloxacillin (83%, 30/36) and amoxicillin-clavulanic acid (69%, 25/36). Only 3% (1/36) were AmpC producers, 42% (15/36) showed a possible mutation in gyrA and 14% (5/36) at least two possible mutations in gyrA or gyrA+parC. In addition, 33% (12/36) showed high probabilities of presence of qnr genes. The enzymes associated to aminoglycoside resistance mechanism were positive in 39% (14/36) to AAC (6’), 28% (10/36) to ANT (2") and 11% (4/36) to AAC (3) IV.

AB - The aim of this study was to detect the antimicrobial resistance mechanisms of 36 Escherichia coli isolates to beta-lactams, quinolones and aminoglycosides using the Kirby-Bauer technique. Thirty-six E. coli isolates from pigs of technified production farms obtained during the 2010-2015 period were used. Fifteen antimicrobials of importance in human and veterinary medicine were tested. The isolates showed resistance mainly to nalidixic acid (89%, 32/36), cloxacillin (83%, 30/36) and amoxicillin-clavulanic acid (69%, 25/36). Only 3% (1/36) were AmpC producers, 42% (15/36) showed a possible mutation in gyrA and 14% (5/36) at least two possible mutations in gyrA or gyrA+parC. In addition, 33% (12/36) showed high probabilities of presence of qnr genes. The enzymes associated to aminoglycoside resistance mechanism were positive in 39% (14/36) to AAC (6’), 28% (10/36) to ANT (2") and 11% (4/36) to AAC (3) IV.

KW - Antimicrobial resistance

KW - Escherichia coli

KW - Pigs

KW - Public health

KW - Resistance mechanisms

UR - http://www.scopus.com/inward/record.url?scp=85065606981&partnerID=8YFLogxK

U2 - 10.15381/rivep.v30i1.15670

DO - 10.15381/rivep.v30i1.15670

M3 - Artículo

AN - SCOPUS:85065606981

SN - 1682-3419

VL - 30

SP - 455

EP - 464

JO - Revista de Investigaciones Veterinarias del Peru

JF - Revista de Investigaciones Veterinarias del Peru

IS - 1

ER -

Detección fenotípica de mecanismos de resistencia antimicrobiana de Escherichia coli aisladas de infecciones entéricas de porcinos provenientes de granjas de producción tecnificada

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