Detection of Helicobacter pylori in gastric cancer tissue through histopathology, immunohistochemistry and real-time reverse transcription-PCR

Carlos A Castaneda, Miluska Castillo, Joselyn Sanchez, Sandro Casavilca, Juvenal Sanchez, Luis A Bernabe, Nancy Suarez, Ivan Chavez, Eloy Ruiz, Katherine Tello, Maria R Villa, Rocio Zevallos, Paola Montenegro, Emmanuel Dias-Neto, Maria Landa-Baella, Luis Taxa

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Aim: Helicobacter pylori is usually detected based on hematoxylin-eosin (H-E) features, but, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are more precise in chronic-gastritis. We evaluated the relevance of these tests in Peruvian gastric cancer samples. Materials & methods: We performed and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides were independently evaluated by three pathologists. Results: H. pylori was detected in 167/287 through H-E, 140/288 through IHC and 175/288 through RT-PCR, and positive-status were associated (p < 0.001). H. pylori detection by H-E had a good concordance with IHC (kappa index = 0.632) but poor with RT-PCR (kappa index = 0.317). Higher median gene-copies were found in high H. pylori density through H-E or IHC (p < 0.001). Conclusion: H-E evaluation is accurate in gastric cancer, and IHC and RT-PCR can complement its results.

Original languageEnglish
Pages (from-to)1131-1137
Number of pages7
JournalFuture Microbiology
Volume15
Issue number12
DOIs
StatePublished - Aug 2020
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by CONCYTEC, under contracts 197-2015-FONDECYT and 204-2015-FONDECYT. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

Publisher Copyright:
© 2020 Future Medicine Ltd.. All rights reserved.

Keywords

  • Helicobacter pylori
  • PCR
  • eosin
  • hematoxylin
  • histopathology
  • immunohistochemistry
  • stomach neoplasms

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