Detección del gen ermB asociado a la resistencia a macrólidos en cepas de Campylobacter aisladas de pollos comercializados en Lima, Perú

Translated title of the contribution: Detection of the ermB gene associated with macrolide resistance in Campylobacter strains isolated from chickens marketed in Lima, Peru

Diego Anampa, Christian Benites, César Lázaro, Juan Espinoza, Pedro Angulo, Diego Díaz, Alberto Manchego, Miguel Rojas

Research output: Contribution to journalArticlepeer-review

Abstract

Objective. To detect the presence of the ermB gene associated with macrolide resistance in Campylobacter spp. strains isolated from chickens marketed in Lima, Peru. Methods. 120 samples of chicken skin from three markets in the districts of San Martin de Porres (n = 30), Santa Anita (n = 20), and Independencia (n = 70), located in the Province of Lima, Peru, were analyzed. Microbiological analysis of the samples was carried out according to ISO standard 10272-1:2017. For the polymerase chain reaction (PCR) confirmation of genus and species, 16-rRNA and GlyA and hipO primers, respectively, were used. For the evaluation of antibiotic sensitivity, the Müller-Hinton agar with 5% blood, with sensi-discs for azithromycin (15 µg) and erythromycin (15 µg), was used. For detection of the ermB gene in strains with resistant phenotypes, conventional PCR was used. Results. A total of 117 positive samples (97.5%) were obtained; of these, 100% were compatible with Campylobacter coli (negative hippurate test) and confirmed by PCR. The plate-based assessment of antibiotic resistance to azithromycin and erythromycin resulted in 100% of strains with a phenotype that is resistant to these macrolides, while the PCR to detect the ermB gene indicated a total of 62 positives (53%), which were confirmed through sequencing. Conclusions. These results demonstrate that the chicken carcasses sold in markets in Lima present contamination by C. coli with high resistance to macrolides, which can be attributed to the presence of the ermB gene.

Translated title of the contributionDetection of the ermB gene associated with macrolide resistance in Campylobacter strains isolated from chickens marketed in Lima, Peru
Original languageSpanish
JournalRevista Panamericana de Salud Publica/Pan American Journal of Public Health
Volume44
Issue number1
DOIs
StatePublished - Sep 2020

Bibliographical note

Publisher Copyright:
© 2020 Pan American Health Organization. All rights reserved.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

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