Detección del gen PrP de scrapie en ovinos junín

Translated title of the contribution: Detection of the PrP gene in junin sheep

Rivera J. Kamilo, Maturrano H. Lenin, Manuel Aguilar Juan, Rosadio A. Raúl

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to establish the frequency of resistance and susceptible genotypes to scrapie in the Junin sheep breed. For this, 56 blood samples were collected in a sheep farm in the central highlands of Peru. The DNA was extracted and the PCR products were analyzed by enzymatic digestion using restriction endonucleases that recognize the PrP gene polymorphisms. Four of the 5 haplotypes reported for the PrP gene were identified: ARR (48.2%), ARQ (35.7%), AHQ (10.7%) and VRQ (5.4%). In addition, 7 of the 15 reported genotypes for the Prion gene were found: ARR/ARR (17.9%), ARR/AHQ (10.7%), ARR/ARQ (42.9%), ARQ/AHQ (10.7%), ARQ/ARQ (7.1%), ARR/ VRQ (7.1%) and ARQ/VRQ (3.6%). Finally, 82.1% of the animals had genotypes resistant to the disease and 17.9% showed genotypes of susceptibility.

Translated title of the contributionDetection of the PrP gene in junin sheep
Original languageSpanish
Pages (from-to)57-65
Number of pages9
JournalRevista de Investigaciones Veterinarias del Peru
Volume26
Issue number1
DOIs
StatePublished - 2015
Externally publishedYes

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