TY - JOUR
T1 - Development and evaluation of a magnetic immunochromatographic test to detect taenia solium, which causes taeniasis and neurocysticercosis in humans
AU - Handali, Sukwan
AU - Klarman, Molly
AU - Gaspard, Amanda N.
AU - Dong, X. Fan
AU - LaBorde, Ronald
AU - Noh, John
AU - Lee, Yeuk Mui
AU - Rodriguez, Silvia
AU - Gonzalez, Armando E.
AU - H.Garcia, Hector
AU - Gilman, Robert H.
AU - Tsang, Victor C.W.
AU - Wilkins, Patricia P.
PY - 2010/4/1
Y1 - 2010/4/1
N2 - Taeniasis/cysticercosis caused by Taenia solium is a frequent parasitic infection of the human brain in most of the world. Rapid and simple screening tools to identify taeniasis and cysticercosis cases are needed for control programs, mostly to identify tapeworm carriers which are the source of infection and need to be treated, or as tools for point-of-care case detection or confirmation. These screening assays should be affordable, reliable, rapid, and easy to perform. Immunochromatographic tests meet these criteria. To demonstrate proof of principle, we developed and evaluated two magnetic immunochromatographic tests (MICTs) for detection of human Taenia solium taeniasis antibodies (ES33-MICT) and neurocysticercosis antibodies (T24-MICT). These assays detected stage-specific antibodies by using two recombinant proteins, rES33 for detection of taeniasis antibodies and rT24H for detection of cysticercosis antibodies. The sensitivity and specificity of the ES33-MICT to detect taeniasis infections were 94.5% and 96%, respectively, and those of the T24-MICT to detect cases of human cysticercosis with two or more viable brain cysts were 93.9% and 98.9%, respectively. These data provide proof of principle that the ES33-and T24-MICTs provide rapid and suitable methods to identify individuals with taeniasis and cysticercosis. Copyright © 2010, American Society for Microbiology. All Rights Reserved. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
AB - Taeniasis/cysticercosis caused by Taenia solium is a frequent parasitic infection of the human brain in most of the world. Rapid and simple screening tools to identify taeniasis and cysticercosis cases are needed for control programs, mostly to identify tapeworm carriers which are the source of infection and need to be treated, or as tools for point-of-care case detection or confirmation. These screening assays should be affordable, reliable, rapid, and easy to perform. Immunochromatographic tests meet these criteria. To demonstrate proof of principle, we developed and evaluated two magnetic immunochromatographic tests (MICTs) for detection of human Taenia solium taeniasis antibodies (ES33-MICT) and neurocysticercosis antibodies (T24-MICT). These assays detected stage-specific antibodies by using two recombinant proteins, rES33 for detection of taeniasis antibodies and rT24H for detection of cysticercosis antibodies. The sensitivity and specificity of the ES33-MICT to detect taeniasis infections were 94.5% and 96%, respectively, and those of the T24-MICT to detect cases of human cysticercosis with two or more viable brain cysts were 93.9% and 98.9%, respectively. These data provide proof of principle that the ES33-and T24-MICTs provide rapid and suitable methods to identify individuals with taeniasis and cysticercosis. Copyright © 2010, American Society for Microbiology. All Rights Reserved. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77950662479&origin=inward
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=77950662479&origin=inward
U2 - 10.1128/CVI.00511-09
DO - 10.1128/CVI.00511-09
M3 - Article
SN - 1556-6811
SP - 631
EP - 637
JO - Clinical and Vaccine Immunology
JF - Clinical and Vaccine Immunology
ER -