Development and evaluation of porcine cysticercosis quickelisa in triturus® EIA analyzer

Sukwan Handali, Sowmya Pattabhi, Yeuk Mui Lee, Maria Silva-Ibanez, Victor A. Kovalenko, Andrew E. Levin, Armando E. Gonzalez, Jacquelin M. Roberts, Hector H. Garcia, Robert H. Gilman, Kathy Hancock, Victor C.W. Tsang

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4 Scopus citations

Abstract

We evaluated three diagnostic antigens (recombinant GP50, recombinant T24H, and synthetic Ts18var1) for cysticercosis and found that all three performed well in detecting cysticercosis in humans and pigs in several assay formats. These antigens were adapted to a new antibody detection format (QuickELISA). With one single incubation step which involves all reactants except the enzyme substrate, the QuickELISA is particularly suited for automation. We formatted the QuickELISA for the Triturus EIA analyzer for testing large numbers of samples. We found that in QuickELISA formats rGP50 and rT24H have better sensitivity and specificity than sTs18var1 for detecting porcine cysticercosis.
Original languageAmerican English
Pages (from-to)60-70
Number of pages11
JournalJournal of Immunoassay and Immunochemistry
DOIs
StatePublished - 1 Jan 2010

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    Handali, S., Pattabhi, S., Lee, Y. M., Silva-Ibanez, M., Kovalenko, V. A., Levin, A. E., Gonzalez, A. E., Roberts, J. M., Garcia, H. H., Gilman, R. H., Hancock, K., & Tsang, V. C. W. (2010). Development and evaluation of porcine cysticercosis quickelisa in triturus® EIA analyzer. Journal of Immunoassay and Immunochemistry, 60-70. https://doi.org/10.1080/15321810903405068