Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy

Christina M. Scheel; Sukwan Handali; Edneia C. Bueno; Azra Khan; Kathy Hancock; Armando E. Gonzalez; Hector H. Garcia; Robert H. Gilman; Victor C.W. Tsang

doi:10.1080/15321810600573028

Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy

Christina M. Scheel, Sukwan Handali, Edneia C. Bueno, Azra Khan, Kathy Hancock, Armando E. Gonzalez, Hector H. Garcia, Robert H. Gilman, Victor C.W. Tsang

Research output: Contribution to journal › Article › peer-review

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Abstract

Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5°C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development. Copyright © Taylor & Francis Group, LLC.

Original language	American English
Pages (from-to)	173-181
Number of pages	9
Journal	Journal of Immunoassay and Immunochemistry
DOIs	https://doi.org/10.1080/15321810600573028
State	Published - 1 Jul 2006

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Scheel, C. M., Handali, S., Bueno, E. C., Khan, A., Hancock, K., Gonzalez, A. E., Garcia, H. H., Gilman, R. H., & Tsang, V. C. W. (2006). Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy. Journal of Immunoassay and Immunochemistry, 173-181. https://doi.org/10.1080/15321810600573028

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title = "Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy",

abstract = "Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5°C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development. Copyright {\textcopyright} Taylor & Francis Group, LLC.",

author = "Scheel, {Christina M.} and Sukwan Handali and Bueno, {Edneia C.} and Azra Khan and Kathy Hancock and Gonzalez, {Armando E.} and Garcia, {Hector H.} and Gilman, {Robert H.} and Tsang, {Victor C.W.}",

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AU - Scheel, Christina M.

AU - Handali, Sukwan

AU - Bueno, Edneia C.

AU - Khan, Azra

AU - Hancock, Kathy

AU - Gonzalez, Armando E.

AU - Garcia, Hector H.

AU - Gilman, Robert H.

AU - Tsang, Victor C.W.

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AB - Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5°C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development. Copyright © Taylor & Francis Group, LLC.

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Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: Use for comparison of antigen efficacy

Abstract

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