Diagnostic accuracy of somatic and excretory-secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis

William Henry Roldán Gonzáles; Dirce Mary Correia Lima Meisel; Fabiana Martins de Paula; Ronaldo Cesar Borges Gryschek

doi:10.1017/S0031182021001207

Diagnostic accuracy of somatic and excretory-secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis

William Henry Roldán Gonzáles, Dirce Mary Correia Lima Meisel, Fabiana Martins de Paula, Ronaldo Cesar Borges Gryschek

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

To evaluate the diagnostic accuracy of three types of antigenic preparations from Strongyloides venezuelensis infective larvae for detection of serum IgG anti-Strongyloides antibodies by enzyme-linked immunosorbent assay (ELISA). Soluble somatic fractions (SSF) and membrane somatic fractions (MSF) and excretory-secretory (E/S) products from S. venezuelensis infective larvae were evaluated against 71 sera from individuals with strongyloidiasis, 105 sera from healthy individuals, and 84 sera from individuals with other helminth infections. Using an ELISA cut-off for 100% sensitivity, E/S products were 97.88% specific followed by MSF (93.12%) and then by SSF (85.2%). The occurrence of cross-reactivity with other helminths was 4.76% (4/84) with E/S products, 8.33% (7/84) with MSF, and 17.86% (15/84) with SSF. For a cut-off for 100% specificity, E/S products showed a sensitivity of 88.73% whereas MSF and SSF showed sensitivities of 59.15% and 53.52%, respectively. In conclusion, E/S products were the best antigenic option for the serodiagnosis of human strongyloidiasis.

Original language	English
Pages (from-to)	1522-1527
Number of pages	6
Journal	Parasitology
Volume	148
Issue number	12
DOIs	https://doi.org/10.1017/S0031182021001207
State	Published - Oct 2021
Externally published	Yes

Bibliographical note

Publisher Copyright:
© The Author(s), 2021. Published by Cambridge University Press.

Keywords

Antibody
Strongyloides
antigen
cross-reactivity
serodiagnosis
strongyloidiasis

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10.1017/S0031182021001207

Cite this

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title = "Diagnostic accuracy of somatic and excretory-secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis",

abstract = "To evaluate the diagnostic accuracy of three types of antigenic preparations from Strongyloides venezuelensis infective larvae for detection of serum IgG anti-Strongyloides antibodies by enzyme-linked immunosorbent assay (ELISA). Soluble somatic fractions (SSF) and membrane somatic fractions (MSF) and excretory-secretory (E/S) products from S. venezuelensis infective larvae were evaluated against 71 sera from individuals with strongyloidiasis, 105 sera from healthy individuals, and 84 sera from individuals with other helminth infections. Using an ELISA cut-off for 100% sensitivity, E/S products were 97.88% specific followed by MSF (93.12%) and then by SSF (85.2%). The occurrence of cross-reactivity with other helminths was 4.76% (4/84) with E/S products, 8.33% (7/84) with MSF, and 17.86% (15/84) with SSF. For a cut-off for 100% specificity, E/S products showed a sensitivity of 88.73% whereas MSF and SSF showed sensitivities of 59.15% and 53.52%, respectively. In conclusion, E/S products were the best antigenic option for the serodiagnosis of human strongyloidiasis.",

keywords = "Antibody, Strongyloides, antigen, cross-reactivity, serodiagnosis, strongyloidiasis",

author = "{Rold{\'a}n Gonz{\'a}les}, {William Henry} and Meisel, {Dirce Mary Correia Lima} and {de Paula}, {Fabiana Martins} and Gryschek, {Ronaldo Cesar Borges}",

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doi = "10.1017/S0031182021001207",

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Diagnostic accuracy of somatic and excretory-secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis. / Roldán Gonzáles, William Henry; Meisel, Dirce Mary Correia Lima; de Paula, Fabiana Martins et al.
In: Parasitology, Vol. 148, No. 12, 10.2021, p. 1522-1527.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Diagnostic accuracy of somatic and excretory-secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis

AU - Roldán Gonzáles, William Henry

AU - Meisel, Dirce Mary Correia Lima

AU - de Paula, Fabiana Martins

AU - Gryschek, Ronaldo Cesar Borges

N1 - Publisher Copyright: © The Author(s), 2021. Published by Cambridge University Press.

PY - 2021/10

Y1 - 2021/10

N2 - To evaluate the diagnostic accuracy of three types of antigenic preparations from Strongyloides venezuelensis infective larvae for detection of serum IgG anti-Strongyloides antibodies by enzyme-linked immunosorbent assay (ELISA). Soluble somatic fractions (SSF) and membrane somatic fractions (MSF) and excretory-secretory (E/S) products from S. venezuelensis infective larvae were evaluated against 71 sera from individuals with strongyloidiasis, 105 sera from healthy individuals, and 84 sera from individuals with other helminth infections. Using an ELISA cut-off for 100% sensitivity, E/S products were 97.88% specific followed by MSF (93.12%) and then by SSF (85.2%). The occurrence of cross-reactivity with other helminths was 4.76% (4/84) with E/S products, 8.33% (7/84) with MSF, and 17.86% (15/84) with SSF. For a cut-off for 100% specificity, E/S products showed a sensitivity of 88.73% whereas MSF and SSF showed sensitivities of 59.15% and 53.52%, respectively. In conclusion, E/S products were the best antigenic option for the serodiagnosis of human strongyloidiasis.

AB - To evaluate the diagnostic accuracy of three types of antigenic preparations from Strongyloides venezuelensis infective larvae for detection of serum IgG anti-Strongyloides antibodies by enzyme-linked immunosorbent assay (ELISA). Soluble somatic fractions (SSF) and membrane somatic fractions (MSF) and excretory-secretory (E/S) products from S. venezuelensis infective larvae were evaluated against 71 sera from individuals with strongyloidiasis, 105 sera from healthy individuals, and 84 sera from individuals with other helminth infections. Using an ELISA cut-off for 100% sensitivity, E/S products were 97.88% specific followed by MSF (93.12%) and then by SSF (85.2%). The occurrence of cross-reactivity with other helminths was 4.76% (4/84) with E/S products, 8.33% (7/84) with MSF, and 17.86% (15/84) with SSF. For a cut-off for 100% specificity, E/S products showed a sensitivity of 88.73% whereas MSF and SSF showed sensitivities of 59.15% and 53.52%, respectively. In conclusion, E/S products were the best antigenic option for the serodiagnosis of human strongyloidiasis.

KW - Antibody

KW - Strongyloides

KW - antigen

KW - cross-reactivity

KW - serodiagnosis

KW - strongyloidiasis

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DO - 10.1017/S0031182021001207

M3 - Artículo

AN - SCOPUS:85109506604

SN - 0031-1820

VL - 148

SP - 1522

EP - 1527

JO - Parasitology

JF - Parasitology

IS - 12

ER -

Diagnostic accuracy of somatic and excretory-secretory antigens from Strongyloides venezuelensis infective larvae for the immunodiagnosis of human strongyloidiasis

Abstract

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Keywords

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