Diphyllobothrium sprakeri n. sp. (Cestoda: Diphyllobothriidae): a hidden broad tapeworm from sea lions off North and South America

Jesús S. Hernández-Orts, Tetiana A. Kuzmina, Luis A. Gomez-Puerta, Roman Kuchta

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The systematic of several marine diphyllobothriid tapeworms of pinnipeds has been revised in recent years. However, 20 species of Diphyllobothrium from phocids and otariids are still recognized as incertae sedis. We describe a new species of Diphyllobothrium from the intestine of California sea lions Zalophus californianus (Lesson) (type-host) and South American sea lions Otaria flavescens (Shaw). Methods: Zalophus californianus from the Pacific coast of the USA and O. flavescens from Peru and Argentina were screened for parasites. Partial fragments of the large ribosomal subunit gene (lsrDNA) and the cytochrome c oxidase subunit 1 (cox1) mitochondrial gene were amplified for 22 isolates. Properly fixed material from California sea lions was examined using light and scanning electron microscopy. Results: A total of four lsrDNA and 21 cox1 sequences were generated and aligned with published sequences of other diphyllobothriid taxa. Based on cox1 sequences, four diphyllobothriid tapeworms from O. flavescens in Peru were found to be conspecific with Adenocephalus pacificus Nybelin, 1931. The other newly generated sequences fall into a well-supported clade with sequences of a putative new species previously identified as Diphyllobothrium sp. 1. from Z. californianus and O. flavescens. A new species, Diphyllobothrium sprakeri n. sp., is proposed for tapeworms of this clade. Conclusions: Diphyllobothrium sprakeri n. sp. is the first diphyllobothriid species described from Z. californianus from the Pacific coast of North America, but O. flavescens from Argentina, Chile and Peru was confirmed as an additional host. The present study molecularly confirmed the first coinfection of two diphyllobothriid species in sea lions from the Southern Hemisphere.[Figure not available: see fulltext.]

Original languageEnglish
Article number219
JournalParasites and Vectors
Volume14
Issue number1
DOIs
StatePublished - Dec 2021

Bibliographical note

Funding Information:
This study was partially supported by the joint research project "Helminth parasites in aquatic ecosystems: their diversity and life-cycles in the changing world" between the National Academy of Science of Ukraine and the Czech Academy of Sciences (2013–2016). JSH-O benefited from a postdoctoral fellowship of the National Council of Science and Technology (CONACYT) of Mexico (Postdoctoral Number. 177603).

Funding Information:
The authors thank Frances Gulland, Christine Fontaine, Barbie Halaska,?Padraig Duignan and other colleagues from the Marine Mammal Center, Sausalito, California, USA, for their help performing the parasitological examination of dead CSL and providing some specimens of cestodes. We also thank the staff of the Laboratorio de Mam?feros Marinos, Centro para el Estudio de Sistemas Marinos (CESIMAR ? CCT CONICET ? CENPAT), Puerto Madryn, Argentina, for their assistance with the examination of stranded SASL. The SASL from Peru was kindly donated by the National Forest Service and Wildlife (SERFOR) of Peru. We also thank Jan Brabec (Institute of Parasitology, Czech Republic) for providing some new sequences for this study.

Publisher Copyright:
© 2021, The Author(s).

Keywords

  • Helminths
  • Otaria flavescens
  • Otariidae
  • Parasites
  • Pinnipedia
  • Zalophus californianus
  • cox1
  • lsrDNA

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