TY - JOUR
T1 - Discovery of diverse polyomaviruses in bats and the evolutionary history of the Polyomaviridae
AU - Tao, Ying
AU - Shi, Mang
AU - Conrardy, Christina
AU - Kuzmin, Ivan V.
AU - Recuenco Cabrera, Sergio Eli
AU - Agwanda, Bernard
AU - Alvarez, Danilo A.
AU - Ellison, James A.
AU - Gilbert, Amy T.
AU - Moran, David
AU - Niezgoda, Michael
AU - Lindblade, Kim A.
AU - Holmes, Edward C.
AU - Breiman, Robert F.
AU - Rupprecht, Charles E.
AU - Tong, Suxiang
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/4
Y1 - 2013/4
N2 - Polyomaviruses (PyVs) have been identified in a wide range of avian and mammalian species. However, little is known about their occurrence, genetic diversity and evolutionary history in bats, even though bats are important reservoirs for many emerging viral pathogens. This study screened 380 specimens from 35 bat species from Kenya and Guatemala for the presence of PyVs by semi-nested pan-PyV PCR assays. PyV DNA was detected in 24 of the 380 bat specimens. Phylogenetic analysis revealed that the bat PyV sequences formed 12 distinct lineages. Fullgenome sequences were obtained for seven representative lineages and possessed similar genomic features to known PyVs. Strikingly, this evolutionary analysis revealed that the bat PyVs were paraphyletic, suggestive of multiple species jumps between bats and other mammalian species, such that the theory of virus-host co-divergence for mammalian PyVs as a whole could be rejected. In addition, evidence was found for strong heterogeneity in evolutionary rate and potential recombination in a number of PyV complete genomes, which complicates both phylogenetic analysis and virus classification. In summary, this study revealed that bats are important reservoirs of PyVs and that these viruses have a complex evolutionary history.
AB - Polyomaviruses (PyVs) have been identified in a wide range of avian and mammalian species. However, little is known about their occurrence, genetic diversity and evolutionary history in bats, even though bats are important reservoirs for many emerging viral pathogens. This study screened 380 specimens from 35 bat species from Kenya and Guatemala for the presence of PyVs by semi-nested pan-PyV PCR assays. PyV DNA was detected in 24 of the 380 bat specimens. Phylogenetic analysis revealed that the bat PyV sequences formed 12 distinct lineages. Fullgenome sequences were obtained for seven representative lineages and possessed similar genomic features to known PyVs. Strikingly, this evolutionary analysis revealed that the bat PyVs were paraphyletic, suggestive of multiple species jumps between bats and other mammalian species, such that the theory of virus-host co-divergence for mammalian PyVs as a whole could be rejected. In addition, evidence was found for strong heterogeneity in evolutionary rate and potential recombination in a number of PyV complete genomes, which complicates both phylogenetic analysis and virus classification. In summary, this study revealed that bats are important reservoirs of PyVs and that these viruses have a complex evolutionary history.
UR - http://www.scopus.com/inward/record.url?scp=84875682552&partnerID=8YFLogxK
U2 - 10.1099/vir.0.047928-0
DO - 10.1099/vir.0.047928-0
M3 - Artículo
C2 - 23239573
AN - SCOPUS:84875682552
VL - 94
SP - 738
EP - 748
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - PART4
ER -