Distribution of tumor-infiltrating immune cells in glioblastoma

Enrique Orrego; Carlos A. Castaneda; Miluska Castillo; Luis A. Bernabe; Sandro Casavilca; Arnab Chakravarti; Wei Meng; Pamela Garcia-Corrochano; Maria R. Villa-Robles; Rocio Zevallos; Omar Mejia; Pedro Deza; Carolina Belmar-Lopez; Luis Ojeda

doi:10.2217/cns-2017-0037

Distribution of tumor-infiltrating immune cells in glioblastoma

Enrique Orrego, Carlos A. Castaneda, Miluska Castillo, Luis A. Bernabe, Sandro Casavilca, Arnab Chakravarti, Wei Meng, Pamela Garcia-Corrochano, Maria R. Villa-Robles, Rocio Zevallos, Omar Mejia, Pedro Deza, Carolina Belmar-Lopez, Luis Ojeda

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Aim: Evaluation of features related to infiltrating immune cell level in glioblastoma. Methods: Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis. Results: CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4⁺ was associated with unmethylated MGMT (p = 0.016). Higher CD8⁺ was associated with larger tumoral size (p = 0.027). Higher CD163⁺ was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4⁺ (p < 0.05) were associated with longer overall survival. Conclusion: Macrophages are more frequent than TILs. Some subsets are associated with clinical features.

Original language	English
Article number	0037
Journal	CNS oncology
Volume	7
Issue number	4
DOIs	https://doi.org/10.2217/cns-2017-0037
State	Published - 2018

Bibliographical note

Publisher Copyright:
© 2018 2018 Orrego, Castaneda, Castillo.

Keywords

MGMT
biomarker
glioblastoma
macrophages
overall survival
prognosis
tumor-infiltrating lymphocytes

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title = "Distribution of tumor-infiltrating immune cells in glioblastoma",

abstract = "Aim: Evaluation of features related to infiltrating immune cell level in glioblastoma. Methods: Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis. Results: CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4+ was associated with unmethylated MGMT (p = 0.016). Higher CD8+ was associated with larger tumoral size (p = 0.027). Higher CD163+ was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4+ (p < 0.05) were associated with longer overall survival. Conclusion: Macrophages are more frequent than TILs. Some subsets are associated with clinical features.",

keywords = "MGMT, biomarker, glioblastoma, macrophages, overall survival, prognosis, tumor-infiltrating lymphocytes",

author = "Enrique Orrego and Castaneda, {Carlos A.} and Miluska Castillo and Bernabe, {Luis A.} and Sandro Casavilca and Arnab Chakravarti and Wei Meng and Pamela Garcia-Corrochano and Villa-Robles, {Maria R.} and Rocio Zevallos and Omar Mejia and Pedro Deza and Carolina Belmar-Lopez and Luis Ojeda",

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volume = "7",

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T1 - Distribution of tumor-infiltrating immune cells in glioblastoma

AU - Orrego, Enrique

AU - Castaneda, Carlos A.

AU - Castillo, Miluska

AU - Bernabe, Luis A.

AU - Casavilca, Sandro

AU - Chakravarti, Arnab

AU - Meng, Wei

AU - Garcia-Corrochano, Pamela

AU - Villa-Robles, Maria R.

AU - Zevallos, Rocio

AU - Mejia, Omar

AU - Deza, Pedro

AU - Belmar-Lopez, Carolina

AU - Ojeda, Luis

PY - 2018

Y1 - 2018

N2 - Aim: Evaluation of features related to infiltrating immune cell level in glioblastoma. Methods: Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis. Results: CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4+ was associated with unmethylated MGMT (p = 0.016). Higher CD8+ was associated with larger tumoral size (p = 0.027). Higher CD163+ was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4+ (p < 0.05) were associated with longer overall survival. Conclusion: Macrophages are more frequent than TILs. Some subsets are associated with clinical features.

AB - Aim: Evaluation of features related to infiltrating immune cell level in glioblastoma. Methods: Tumor-infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis. Results: CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4+ was associated with unmethylated MGMT (p = 0.016). Higher CD8+ was associated with larger tumoral size (p = 0.027). Higher CD163+ was associated with higher age (p = 0.044) and recursive partitioning analysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4+ (p < 0.05) were associated with longer overall survival. Conclusion: Macrophages are more frequent than TILs. Some subsets are associated with clinical features.

KW - MGMT

KW - biomarker

KW - glioblastoma

KW - macrophages

KW - overall survival

KW - prognosis

KW - tumor-infiltrating lymphocytes

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DO - 10.2217/cns-2017-0037

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AN - SCOPUS:85062250676

SN - 2045-0907

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JO - CNS oncology

JF - CNS oncology

IS - 4

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ER -

Distribution of tumor-infiltrating immune cells in glioblastoma

Abstract

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Keywords

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