Diversity of astacin-like metalloproteases identified by transcriptomic analysis in Peruvian Loxosceles laeta spider venom and in vitro activity characterization

Raíssa Medina-Santos; Clara Guerra-Duarte; Sabrina de Almeida Lima; Fernanda Costal-Oliveira; Priscilla Alves de Aquino; Anderson Oliveira do Carmo; César  Bonilla-Ferreyra; Edgar E. Gonzalez-Kozlova; Evanguedes Kalapothakis; Carlos Chávez-Olórtegui

doi:10.1016/j.biochi.2019.08.017

Diversity of astacin-like metalloproteases identified by transcriptomic analysis in Peruvian Loxosceles laeta spider venom and in vitro activity characterization

Raíssa Medina-Santos, Clara Guerra-Duarte, Sabrina de Almeida Lima, Fernanda Costal-Oliveira, Priscilla Alves de Aquino, Anderson Oliveira do Carmo, César Bonilla-Ferreyra, Edgar E. Gonzalez-Kozlova, Evanguedes Kalapothakis, Carlos Chávez-Olórtegui

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Loxosceles spiders are found in almost all countries of South America. In Peru, Loxosceles laeta species is the main responsible for the accidents caused by poisonous animals, being known as “killer spiders”, due to the large number of fatal accidents observed. Astacin-like metalloproteases, named LALPs (Loxosceles astacin-like metalloproteases) are highly expressed in Loxosceles spiders venom gland. These proteases may be involved in hemorrhage and venom spreading, being relevant to the envenoming proccess. Thus, the aim of this work was to analyze Peruvian L. laeta venom gland transcripts using bioinformatics tools, focusing on LALPs. A cDNA library from Peruvian L. laeta venom glands was constructed and sequenced by MiSeq (Illumina) sequencer. After assembly, the resulting sequences were annotated, seeking out for similarity with previously described LALPs. Nine possible LALPs isoforms from Peruvian L. laeta venom were identified and the results were validated by in silico and in vitro experiments. This study contributes to a better understanding of the molecular diversity of Loxosceles venom and provide insights about the action of LALPs.

Original language	English
Pages (from-to)	81-92
Number of pages	12
Journal	Biochimie
Volume	167
DOIs	https://doi.org/10.1016/j.biochi.2019.08.017
State	Published - Dec 2019

Bibliographical note

Funding Information:
This research was supported by the Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, Brazil (CNPq) (no 17/2013, process: 490269/2013-3), Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, Brazil - CAPES (TOXINOLOGIA no23038000825/2011?63) and by Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG) (no PPM: 00270-16).

Funding Information:
This research was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil ( CNPq ) (no 17/2013 , process: 490269/2013-3 ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil - CAPES ( TOXINOLOGIA no 23038000825/2011–63 ) and by Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Brazil ( FAPEMIG ) (no PPM: 00270-16 ).

Publisher Copyright:
© 2019

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

Keywords

LALP
Loxosceles astacin-like metalloproteases
Loxosceles laeta
Metalloprotease
Spider venom
Transcriptome

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10.1016/j.biochi.2019.08.017

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Medina-Santos, R., Guerra-Duarte, C., de Almeida Lima, S., Costal-Oliveira, F., Alves de Aquino, P., Oliveira do Carmo, A., Bonilla-Ferreyra, C., Gonzalez-Kozlova, E. E., Kalapothakis, E., & Chávez-Olórtegui, C. (2019). Diversity of astacin-like metalloproteases identified by transcriptomic analysis in Peruvian Loxosceles laeta spider venom and in vitro activity characterization. Biochimie, 167, 81-92. https://doi.org/10.1016/j.biochi.2019.08.017

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title = "Diversity of astacin-like metalloproteases identified by transcriptomic analysis in Peruvian Loxosceles laeta spider venom and in vitro activity characterization",

abstract = "Loxosceles spiders are found in almost all countries of South America. In Peru, Loxosceles laeta species is the main responsible for the accidents caused by poisonous animals, being known as “killer spiders”, due to the large number of fatal accidents observed. Astacin-like metalloproteases, named LALPs (Loxosceles astacin-like metalloproteases) are highly expressed in Loxosceles spiders venom gland. These proteases may be involved in hemorrhage and venom spreading, being relevant to the envenoming proccess. Thus, the aim of this work was to analyze Peruvian L. laeta venom gland transcripts using bioinformatics tools, focusing on LALPs. A cDNA library from Peruvian L. laeta venom glands was constructed and sequenced by MiSeq (Illumina) sequencer. After assembly, the resulting sequences were annotated, seeking out for similarity with previously described LALPs. Nine possible LALPs isoforms from Peruvian L. laeta venom were identified and the results were validated by in silico and in vitro experiments. This study contributes to a better understanding of the molecular diversity of Loxosceles venom and provide insights about the action of LALPs.",

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Medina-Santos, R, Guerra-Duarte, C, de Almeida Lima, S, Costal-Oliveira, F, Alves de Aquino, P, Oliveira do Carmo, A, Bonilla-Ferreyra, C, Gonzalez-Kozlova, EE, Kalapothakis, E & Chávez-Olórtegui, C 2019, 'Diversity of astacin-like metalloproteases identified by transcriptomic analysis in Peruvian Loxosceles laeta spider venom and in vitro activity characterization', Biochimie, vol. 167, pp. 81-92. https://doi.org/10.1016/j.biochi.2019.08.017

TY - JOUR

T1 - Diversity of astacin-like metalloproteases identified by transcriptomic analysis in Peruvian Loxosceles laeta spider venom and in vitro activity characterization

AU - Medina-Santos, Raíssa

AU - Guerra-Duarte, Clara

AU - de Almeida Lima, Sabrina

AU - Costal-Oliveira, Fernanda

AU - Alves de Aquino, Priscilla

AU - Oliveira do Carmo, Anderson

AU - Bonilla-Ferreyra, César

AU - Gonzalez-Kozlova, Edgar E.

AU - Kalapothakis, Evanguedes

AU - Chávez-Olórtegui, Carlos

PY - 2019/12

Y1 - 2019/12

N2 - Loxosceles spiders are found in almost all countries of South America. In Peru, Loxosceles laeta species is the main responsible for the accidents caused by poisonous animals, being known as “killer spiders”, due to the large number of fatal accidents observed. Astacin-like metalloproteases, named LALPs (Loxosceles astacin-like metalloproteases) are highly expressed in Loxosceles spiders venom gland. These proteases may be involved in hemorrhage and venom spreading, being relevant to the envenoming proccess. Thus, the aim of this work was to analyze Peruvian L. laeta venom gland transcripts using bioinformatics tools, focusing on LALPs. A cDNA library from Peruvian L. laeta venom glands was constructed and sequenced by MiSeq (Illumina) sequencer. After assembly, the resulting sequences were annotated, seeking out for similarity with previously described LALPs. Nine possible LALPs isoforms from Peruvian L. laeta venom were identified and the results were validated by in silico and in vitro experiments. This study contributes to a better understanding of the molecular diversity of Loxosceles venom and provide insights about the action of LALPs.

AB - Loxosceles spiders are found in almost all countries of South America. In Peru, Loxosceles laeta species is the main responsible for the accidents caused by poisonous animals, being known as “killer spiders”, due to the large number of fatal accidents observed. Astacin-like metalloproteases, named LALPs (Loxosceles astacin-like metalloproteases) are highly expressed in Loxosceles spiders venom gland. These proteases may be involved in hemorrhage and venom spreading, being relevant to the envenoming proccess. Thus, the aim of this work was to analyze Peruvian L. laeta venom gland transcripts using bioinformatics tools, focusing on LALPs. A cDNA library from Peruvian L. laeta venom glands was constructed and sequenced by MiSeq (Illumina) sequencer. After assembly, the resulting sequences were annotated, seeking out for similarity with previously described LALPs. Nine possible LALPs isoforms from Peruvian L. laeta venom were identified and the results were validated by in silico and in vitro experiments. This study contributes to a better understanding of the molecular diversity of Loxosceles venom and provide insights about the action of LALPs.

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KW - Loxosceles astacin-like metalloproteases

KW - Loxosceles laeta

KW - Metalloprotease

KW - Spider venom

KW - Transcriptome

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U2 - 10.1016/j.biochi.2019.08.017

DO - 10.1016/j.biochi.2019.08.017

M3 - Artículo

C2 - 31476328

AN - SCOPUS:85072520566

SN - 0300-9084

VL - 167

SP - 81

EP - 92

JO - Biochimie

JF - Biochimie

ER -

Diversity of astacin-like metalloproteases identified by transcriptomic analysis in Peruvian Loxosceles laeta spider venom and in vitro activity characterization

Abstract

Bibliographical note

Keywords

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