Dual Effects of Resveratrol on Cell Death and Proliferation of Colon Cancer Cells

Álvaro San Hipólito-Luengo, Antonio Alcaide, Mariella Ramos-González, Elena Cercas, Susana Vallejo, Alejandra Romero, Elena Talero, Carlos F. Sánchez-Ferrer, Virginia Motilva, Concepción Peiró

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Colorectal cancer remains a main cause of deaths worldwide, and novel agents are being searched to treat this disease. Polyphenols have emerged as promising therapeutic tools in cancer. Resveratrol (3,5,4′-trihydoxy-trans-stilbene) induces cell death in different tumor cell lines, and it also stimulates the proliferation of specific breast and prostate cancer cell lines. Here, we studied the impact of resveratrol over a 100-fold concentration range on cell death and proliferation of HT-29 colorectal adenocarcinoma cells. After 96 h of treatment, a biphasic pattern was observed. At lower concentrations (1 and 10 μmol/l), resveratrol increased the cell number, as did the polyphenol quercetin. At 50 or 100 μmol/l, resveratrol reduced the cell number and increased the percentage of apoptotic or necrotic cells, thus indicating cytotoxicity. On HCT116 colon cancer cells, however, no proliferative properties of resveratrol were observed. Resveratrol-induced cytotoxicity on HT-29 cells was associated with NADPH oxidase activation and increased levels of histone γH2AX, a marker of DNA damage, paralleled by enhanced sirtuin 6 levels, likely as a repair mechanism. Overall, resveratrol may be an effective tool in anti-tumor chemotherapy. However, since under some conditions it may favor tumor cell growth, appropriate local concentrations must be achieved to minimize unwanted effects of resveratrol.

Original languageEnglish
Pages (from-to)1019-1027
Number of pages9
JournalNutrition and Cancer
Volume69
Issue number7
DOIs
StatePublished - 3 Oct 2017

Bibliographical note

Funding Information:
This study was supported by grants from Plan Nacional de I+D (SAF2014-52762-R) and Proyecto de Excelencia de la Junta de Andaluc?a (P12-AGR-430).

Funding Information:
This study was supported by grants from Plan Nacional de ICD (SAF2014-52762-R) and Proyecto de Excelencia de la Junta de Andalućıa (P12-AGR-430).

Publisher Copyright:
© 2017 Taylor & Francis Group, LLC.

Fingerprint

Dive into the research topics of 'Dual Effects of Resveratrol on Cell Death and Proliferation of Colon Cancer Cells'. Together they form a unique fingerprint.

Cite this