TY - JOUR
T1 - Efecto de la Suplementación de L-Glutamina sobre la Expresión de Genes de la Respuesta Inmune Humoral de la Mucosa Intestinal de Crías de Alpacas (Vicugna pacos)
AU - Emanuel, Bejarano L.
AU - Alberto, Manchego S.
AU - Castro Sanguinetti, Gina Ruth
AU - Giovanni, Pérez G.
AU - Sandoval Chaupe, Nieves Nancy
AU - Ramirez Velasquez, Mercy Gisela
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - This study aimed to determine the effect of L-glutamine on the expression of immunoglobulin A (IgA) and cytokines TGF-β and interleukins IL-4, IL-5, IL-6 and IL-10 in the mucosa of the jejunum of 4-7 days old alpacas. A group of 6 alpacas was orally administered 3.3 mM L-glutamine per kg body weight and a second dose 7 days later. A second group of 7 alpacas received PBS as control. All animals were euthanized 3 days after the treatment and a portion of the jejunum was collected. The messenger RNA (mRNA) was extracted and the reverse transcription (RT) and real-time PCR was done using primers specific for each gene in study. The 2-δδct method was used for the relative quantitation of IgA, TGF-β and interleukins. The TGF-β expressed 3 times more and IgA 18 times more in alpacas treated with L-glutamine than in the controls (p<0.05). The expressions of IL-4, IL-5, IL-6 and IL-10 showed similar levels in the treated and control animals. It is concluded that L-glutamine increases the expression of TGF-β and IgA improving the humoral adaptive immune response of intestinal mucosa in new-born alpacas.
AB - This study aimed to determine the effect of L-glutamine on the expression of immunoglobulin A (IgA) and cytokines TGF-β and interleukins IL-4, IL-5, IL-6 and IL-10 in the mucosa of the jejunum of 4-7 days old alpacas. A group of 6 alpacas was orally administered 3.3 mM L-glutamine per kg body weight and a second dose 7 days later. A second group of 7 alpacas received PBS as control. All animals were euthanized 3 days after the treatment and a portion of the jejunum was collected. The messenger RNA (mRNA) was extracted and the reverse transcription (RT) and real-time PCR was done using primers specific for each gene in study. The 2-δδct method was used for the relative quantitation of IgA, TGF-β and interleukins. The TGF-β expressed 3 times more and IgA 18 times more in alpacas treated with L-glutamine than in the controls (p<0.05). The expressions of IL-4, IL-5, IL-6 and IL-10 showed similar levels in the treated and control animals. It is concluded that L-glutamine increases the expression of TGF-β and IgA improving the humoral adaptive immune response of intestinal mucosa in new-born alpacas.
KW - Glutamine
KW - Interleukins
KW - Mucosal immunity
KW - South American camelids
UR - http://www.scopus.com/inward/record.url?scp=85028851545&partnerID=8YFLogxK
U2 - 10.15381/rivep.v28i2.13078
DO - 10.15381/rivep.v28i2.13078
M3 - Artículo
AN - SCOPUS:85028851545
SN - 1682-3419
VL - 28
SP - 387
EP - 396
JO - Revista de Investigaciones Veterinarias del Peru
JF - Revista de Investigaciones Veterinarias del Peru
IS - 2
ER -