Effect of Alendronate on Bone Microarchitecture in Irradiated Rats With Osteoporosis: Micro-CT and Histomorphometric Analysis

Carolina Cintra Gomes, Deborah Queiroz Freitas, Amanda Maria Medeiros Araújo, Laura Ricardina Ramírez-Sotelo, Fernanda Paula Yamamoto-Silva, Brunno Santos de Freitas Silva, Débora de Melo Távora, Solange Maria Almeida

Research output: Contribution to journalArticle

Abstract

© 2017 American Association of Oral and Maxillofacial Surgeons Purpose: The aim of the present study was to evaluate the effect of alendronate (ALN) on the bone microarchitecture of irradiated rats with estrogen deficiency, using microcomputed tomography (micro-CT) and histomorphometric analysis. Materials and Methods: Forty adult Wistar rats were subjected to ovariectomy and randomly divided into the following groups: control (CON), ALN, irradiated (IRR), and ALN/irradiated (ALN/IRR). Approximately 50 days after ovariectomy, the hind limbs of the rats in the IRR and ALN/IRR groups were irradiated with 15 Gy of x-radiation. The rats were euthanized 7 and 30 days after irradiation. The bone microarchitecture was analyzed using micro-CT and histomorphometry. The bone microarchitecture was evaluated using the Mann-Whitney U test, analysis of variance, and the post hoc Tukey test, with statistical significance set at 5%. Results: Irradiation had increased the thickness of the cortical bone at 7 days (P <.05) and also decreased the number of trabeculae per unit length and increased the average distance between the trabeculae (P <.05) at 30 days. ALN inhibited the deleterious effect of x-radiation, preventing the distance between the trabeculae from increasing and the number of trabeculae per unit length from decreasing (P <.05). Conclusions: The present results have demonstrated that the initial effect of ALN could be positive, because it checked the deleterious action in the bone tissue submitted to x-radiation.
Original languageAmerican English
Pages (from-to)972-981
Number of pages10
JournalJournal of Oral and Maxillofacial Surgery
DOIs
StatePublished - 1 May 2018
Externally publishedYes

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  • Cite this

    Gomes, C. C., Freitas, D. Q., Medeiros Araújo, A. M., Ramírez-Sotelo, L. R., Yamamoto-Silva, F. P., de Freitas Silva, B. S., de Melo Távora, D., & Almeida, S. M. (2018). Effect of Alendronate on Bone Microarchitecture in Irradiated Rats With Osteoporosis: Micro-CT and Histomorphometric Analysis. Journal of Oral and Maxillofacial Surgery, 972-981. https://doi.org/10.1016/j.joms.2017.11.019