TY - JOUR
T1 - Efecto de cuatro ecotipos de Lepidium peruvianum Chacón sobre la producción de óxido nítrico in vitro
AU - Alzamora, Libertad
AU - Alvarez, Evelyn
AU - Torres, Dina
AU - Solís, Hilda
AU - Colona Vallejos, Erasmo Honorio
AU - Quispe, Jenny
AU - Chanco, Magda
N1 - Publisher Copyright:
© 2017 Facultad de Ciencias Biológicas UNMSM.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2007/7
Y1 - 2007/7
N2 - The macrophages play an important role in the innate and adaptative response. Upon appropriate activation, macrophages released a variety of cytotoxic mediators like the Nitric Oxide (NO). The objective was to evaluate the production of NO for murine peritoneal macrophages cultivated with methanol extracts (ME) of different ecotypes of Lepidium peruvianum Chacon (at present Lepidium meyenii Walp.) red, black, purple and white. The ME was prepared using maca powdered macerated in methanol (1:2) during 10 days. Peritoneal macrophages were obtained of mice 3 days after having injected them 1 ml of thioglycolate broth; they were cultivated for triplicate during 18 hrs at 37°C in RPMI 1640 medium supplemented with 10% fetal calf serum. The dose of ME was of 800 μg/ml for ecotype, were considered controls without ME. The production of NO was assayed by nitrite accumulation in the supernatant culture and it was evidenced with Peter Griess's reagent, the nitrite concentrations were calculated based on the standard curve elaborated with NaNO2. The produced concentrations of nitrite were of 7,45; 6,79; 5,76; 5,61 and 6,81 mM for the ME purple, black, white, red ecotypes and control respectively, although with the purple ecotype it was superior (p> 0,05).
AB - The macrophages play an important role in the innate and adaptative response. Upon appropriate activation, macrophages released a variety of cytotoxic mediators like the Nitric Oxide (NO). The objective was to evaluate the production of NO for murine peritoneal macrophages cultivated with methanol extracts (ME) of different ecotypes of Lepidium peruvianum Chacon (at present Lepidium meyenii Walp.) red, black, purple and white. The ME was prepared using maca powdered macerated in methanol (1:2) during 10 days. Peritoneal macrophages were obtained of mice 3 days after having injected them 1 ml of thioglycolate broth; they were cultivated for triplicate during 18 hrs at 37°C in RPMI 1640 medium supplemented with 10% fetal calf serum. The dose of ME was of 800 μg/ml for ecotype, were considered controls without ME. The production of NO was assayed by nitrite accumulation in the supernatant culture and it was evidenced with Peter Griess's reagent, the nitrite concentrations were calculated based on the standard curve elaborated with NaNO2. The produced concentrations of nitrite were of 7,45; 6,79; 5,76; 5,61 and 6,81 mM for the ME purple, black, white, red ecotypes and control respectively, although with the purple ecotype it was superior (p> 0,05).
KW - Activated macrophages
KW - Lepidium meyenii
KW - Lepidium peruvianum
KW - Maca ecotypes
KW - Nitric oxide
UR - http://www.scopus.com/inward/record.url?scp=85044976250&partnerID=8YFLogxK
M3 - Artículo
AN - SCOPUS:85044976250
VL - 13
SP - 215
EP - 217
JO - Revista Peruana de Biologia
JF - Revista Peruana de Biologia
SN - 1561-0837
IS - 3
ER -