Effect of orally administered l-glutamine on gene expression of the humoral immune response of the intestinal mucosa of baby Alpaca (Vicugna Pacos)

Bejarano L. Emanuel, Manchego S. Alberto, Castro S. Gina, Pérez G. Giovanni, Sandoval C. Nieves, Ramírez V. Mercy

Research output: Contribution to journalArticle

Abstract

This study aimed to determine the effect of L-glutamine on the expression of immunoglobulin A (IgA) and cytokines TGF-β and interleukins IL-4, IL-5, IL-6 and IL-10 in the mucosa of the jejunum of 4-7 days old alpacas. A group of 6 alpacas was orally administered 3.3 mM L-glutamine per kg body weight and a second dose 7 days later. A second group of 7 alpacas received PBS as control. All animals were euthanized 3 days after the treatment and a portion of the jejunum was collected. The messenger RNA (mRNA) was extracted and the reverse transcription (RT) and real-time PCR was done using primers specific for each gene in study. The 2-δδct method was used for the relative quantitation of IgA, TGF-β and interleukins. The TGF-β expressed 3 times more and IgA 18 times more in alpacas treated with L-glutamine than in the controls (p<0.05). The expressions of IL-4, IL-5, IL-6 and IL-10 showed similar levels in the treated and control animals. It is concluded that L-glutamine increases the expression of TGF-β and IgA improving the humoral adaptive immune response of intestinal mucosa in new-born alpacas.
Original languageAmerican English
Pages (from-to)387-396
Number of pages10
JournalRevista de Investigaciones Veterinarias del Peru
DOIs
StatePublished - 1 Apr 2017

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