Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders

Alexei Vicent Santiani Acosta, Olger Pedro Ramos Coaguila, Rómulo Sapana, Teodosio Huanca, Néstor Sepúlveda, Raúl Sánchez

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Aim: To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation. Methods: Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were formed: 1) extender I-G; 2) extender I-EG; 3) extender II-G; and 4) extender II-EG. Semen was diluted in a two-step process: for cooling to 5 °C (extenders without CPA), and for freezing (extenders with CPA). Viability and acrosome integrity were assessed using trypan blue and Giemsa stains. Results: When compared, the motility after thawing was higher (P < 0.05) in groups II-EG (20.0% ± 6.7%) and II-G (15.3% ± 4.1%) than that in groups I-G (4.0% ± 1.1%) and I-EG (1.0% ± 1.4%). Viable spermatozoa with intact acrosomes in groups II-EG (18.7% ± 2.9%) and II-G (12.7% ± 5.9%) were higher than that in groups I-G (5.7% ± 1.5%) and I-EG (4.0% ± 1.0%). Conclusion: The skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders.

Original languageEnglish
Pages (from-to)303-309
Number of pages7
JournalAsian Journal of Andrology
Volume7
Issue number3
DOIs
StatePublished - Sep 2005
Externally publishedYes

Keywords

  • Alpaca spermatozoa
  • Cryopreservation
  • Ethylene glycol
  • Extenders
  • Glycerol

Fingerprint Dive into the research topics of 'Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders'. Together they form a unique fingerprint.

Cite this