Evaluation of a di-O-methylated glycan as a potential antigenic target for the serodiagnosis of human toxocariasis

G. R. Elefant, W. H. Roldán, A. Seeböck, P. Kosma

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

© 2016 John Wiley & Sons Ltd. Serodiagnosis of human toxocariasis is based on the detection of specific IgG antibodies by the enzyme-linked immunosorbent assay (ELISA) using Toxocara larvae excretory-secretory (TES) antigens, but its production is a laborious and time-consuming process being also limited by the availability of adult females of T. canis as source for ova to obtain larvae. Chemical synthesis of the di-O-methylated (DiM) glycan structure found in the TES antigens has provided material for studying the antibody reactivity in a range of mammalian hosts, showing reactivity with human IgM and IgG. In this study, we have evaluated the performance of the DiM glycan against a panel of sera including patients with toxocariasis (n = 60), patients with other helminth infections (n = 75) and healthy individuals (n = 94), showing that DiM is able to detect IgG antibodies with a sensitivity and specificity of 91·7% and 94·7%, respectively, with a very good agreement with the TES antigens (kappa = 0·825). However, cross-reactivity was observed in some sera from patients with ascariasis, hymenolepiasis and fascioliasis. These results show that the DiM glycan could be a promising antigenic tool for the serodiagnosis of human toxocariasis.
Original languageAmerican English
Pages (from-to)236-243
Number of pages8
JournalParasite Immunology
DOIs
StatePublished - 1 Apr 2016

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