Evaluación de una técnica de PCR-múltiple para la detección rápida de salmonella typhimurium y enteritidis en cuyes (Cavia porcellus) naturalmente infectados

Translated title of the contribution: Evaluation of a multiplex-PCR method for rapid detection of salmonella typhimurium and enteritidis in naturally infected Guinea pigs (cavia porcellus)

Gerardo Díaz, Rosadio A. Raúl, Marcelo M. Geraldine, Chero O. Ana, Jiménez A. Ronald, Reyna W. Iván, Maturrano H. Lenin

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to evaluate the detection capacity of the Multiplex Polymerase Chain Reaction (PCR) method from non-selective pre-enrichment samples (using target sequences of InvA, fliC and prot6E genes) to diagnose Salmonella Typhimurium and Enteritidis and to determine the concordance between the conventional microbiological detection method which consist of non-selective pre-enrichment, selective enrichment, differential agar isolation and biochemical tests. A total of 111 liver samples from Guinea pigs with presumptive diagnosis of salmonellosis, collected in Chancay (Lima) and El Mantaro (Junín), Peru were analyzed. Salmonella Typhimurium was detected by multiplex PCR in 54% (60/111) of the samples and by microbiological analysis in 41% (45/111). A substantial concordance was found with a Kappa value of 0.64. The McNemar test showed that the results of both tests were statistically different (p<0.05).

Translated title of the contributionEvaluation of a multiplex-PCR method for rapid detection of salmonella typhimurium and enteritidis in naturally infected Guinea pigs (cavia porcellus)
Original languageSpanish
Pages (from-to)713-722
Number of pages10
JournalRevista de Investigaciones Veterinarias del Peru
Volume28
Issue number3
DOIs
StatePublished - 1 Jul 2017

Bibliographical note

Funding Information:
La toma de muestras se llevó a cabo en la estación experimental «El Mantaro» del Instituto Veterinario de Investigaciones de Trópico y Altura (IVITA) de la Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos (FMV-UNMSM) (Jauja, Perú), y en un criadero de crianza comercial en Chancay(Huaral,Perú). El pro-cesamiento de las muestras se llevó a cabo en el laboratorio de Genética y Biología Molecular de la FMV-UNMSM en Lima. Ambas etapas se ejecutaron entre octubre de 2015 y abril de 2016.

Funding Information:
Este estudio fue financiado por el Pro- grama Nacional de Innovación para la Competitividad y Productividad - Innóvate Perú, del Proyecto Contrato 362-PNICP-PIAP-2014 «Desarrollo de una vacuna para el control y prevención de la salmonelosis en la producción cuyes».

Fingerprint Dive into the research topics of 'Evaluation of a multiplex-PCR method for rapid detection of salmonella typhimurium and enteritidis in naturally infected Guinea pigs (cavia porcellus)'. Together they form a unique fingerprint.

Cite this