Evaluation of antimicrobial activity antioxidant and cytotoxicity of ethanolic and aqueous extracts of Tagetes multiflora Kunth chinche

David Velásquez, Teresa Gallardo-Jugo, Mónica G. Retuerto-Figueroa, Eva Ramos-Llica, María Calixto, Celia Vargas De La Cruz, Elizabeth C. Ortega-Romero, Md Shariful Islam

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Introduction: The antimicrobial, antioxidant and cytotoxicity activities of the ethanolic and aqueous extracts of Targets multiflora Kunth were evaluated, which were collected in the province of Yauyos department of Lima-Perú. The antimicrobial activity was evaluated by the well diffusion method on agar against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Method: Pseudomonas aeruginosa ATCC 9027, Bacillus subtilis ATCC 6633, Candida albicans ATCC 14053 and Aspergillus niger ATCC 16404. Antioxidant activity evaluated by the DPPH method and the cytotoxic activity was evaluated by the bioassay of cytotoxicity in Artemia salina (CYTED). In the ethanolic extract, halos were formed against Escherichia coli (11.33 mm ± 0.58), Pseudomonas aeruginosa (13 mm ± 1.0) and Staphyloccocus aureus (15.67 mm ± 1.15) strains; and the aqueous extract against the Aspergillus niger strain (13.67 mm ± 0.58). Results: The Ethanolic extract showed an IC50 of 60.93 μg/mL ± 0.40; and the aqueous extract 40.42 μg/mL ± 0.24. The Ethanolic extract showed a LC50 of 35.568 μg/mL and the aqueous extract 386.048 μg/mL. Conclusion: The Ethanolic extract showed antimicrobial activity (against Escherichia coli, Pseudomonas aeruginosa and Staphyloccocus aureus), antioxidant activity and high cytotoxic activity; while the aqueous extract presented antimicrobial activity (against Aspergillus niger), antioxidant activity and moderate cytotoxic activity.

Original languageEnglish
Pages (from-to)786-800
Number of pages15
JournalAnnals of Tropical Medicine and Public Health
Issue number6
StatePublished - Mar 2020
Externally publishedYes

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  • Antimicrobial
  • Antioxidant
  • Aqueous
  • Cytotoxicity
  • Ethanolic
  • Extract
  • Tagetes multiflora Kunth chinche


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