TY - JOUR
T1 - EXPERIMENTAL BR AIN INFECTION WITH CYSTICERCOSIS IN SHEEP
AU - Sota Ortecho, Katherine Alexandra
AU - Bustos, Javier A.
AU - Verastegui, Manuela R.
AU - Toribio, Luz
AU - Chile, Nancy
AU - Angulo, Noelia
AU - Cangalaya, Carla
AU - Calcina, Juan
AU - González, Armando E.
AU - Gilman, Robert H.
AU - García, Héctor H.
N1 - Publisher Copyright:
© 2022, Instituto Nacional de Salud. All rights reserved.
PY - 2022/9/30
Y1 - 2022/9/30
N2 - Objective. To explore the feasibility of developing a sheep model of neurocysticercosis (NCC) by intracranial infection with T. solium oncospheres. Materials and methods. We carried out an experimental infection model of NCC in sheep. Approximately 10 T. solium oncospheres previously cultured for 30 days were inoculated intracranially into ten sheep. The oncospheres, in 0.1 mL of physiological saline, were injected into the parietal lobe through an 18-gauge needle. Results. After three months, granulo-mas were found in two sheep. In a third sheep we identified a 5 mm diameter cyst in the right lateral ventricle and histological evaluation confirmed that the cyst corresponded to a T. solium larva. Immuno-histochemistry with monoclonal antibodies directed against membrane components and excretory/se-cretory antigens of the T. solium cyst was also used to confirm the etiology of the found granulomas. One of them showed reactivity to the monoclonal antibodies used, thus confirming that it was a cysticercus. Conclusion. This experiment is the proof of concept that it is possible to infect sheep with cysticercosis by intracranial inoculation.
AB - Objective. To explore the feasibility of developing a sheep model of neurocysticercosis (NCC) by intracranial infection with T. solium oncospheres. Materials and methods. We carried out an experimental infection model of NCC in sheep. Approximately 10 T. solium oncospheres previously cultured for 30 days were inoculated intracranially into ten sheep. The oncospheres, in 0.1 mL of physiological saline, were injected into the parietal lobe through an 18-gauge needle. Results. After three months, granulo-mas were found in two sheep. In a third sheep we identified a 5 mm diameter cyst in the right lateral ventricle and histological evaluation confirmed that the cyst corresponded to a T. solium larva. Immuno-histochemistry with monoclonal antibodies directed against membrane components and excretory/se-cretory antigens of the T. solium cyst was also used to confirm the etiology of the found granulomas. One of them showed reactivity to the monoclonal antibodies used, thus confirming that it was a cysticercus. Conclusion. This experiment is the proof of concept that it is possible to infect sheep with cysticercosis by intracranial inoculation.
KW - Cysticercosis source: MeSH NLM)
KW - Epilepsy
KW - Neurocysticercosis
KW - Sheep, Taenia solium
UR - http://www.scopus.com/inward/record.url?scp=85142219245&partnerID=8YFLogxK
U2 - 10.17843/rpmesp.2022.393.11039
DO - 10.17843/rpmesp.2022.393.11039
M3 - Artículo
AN - SCOPUS:85142219245
SN - 1726-4634
VL - 39
SP - 328
EP - 335
JO - Revista Peruana de Medicina Experimental y Salud Publica
JF - Revista Peruana de Medicina Experimental y Salud Publica
IS - 3
ER -
