Abstract
The aim of the study was to determine the relative levels of expression of messenger RNAs of RORγt and IL-17 in intestinal mucosa of clinically healthy newborn alpacas. Three age groups were formed: 2 to 7 days (group 1, n=5), 8 to 20 days (group 2, n=6) and 26 to 47 days (group 3, n=6), regardless sex or breed. Samples were taken from the middle segment of the jejunum. The total RNA was extracted, and the RT-PCR technique was used with specific primers. The relative expression of mRNA of RORγt and IL-17 was determined by the 2-ΔΔCt method using two neonatal pups as calibrators and using GAPDH as an endogenous control. The kinetics of expression of RORγt showed a constant trend during the first and third week of life, reaching its maximum expression in animals from 2 to 7 days of age, unlike IL-17 whose expression was progressive with age, evidencing a high stimulation and expression from the first days of age.
| Translated title of the contribution | Expression and quantification of RORγt and interleukin-17 in intestinal mucosa of newborn alpaca (Vicugna pacos) |
|---|---|
| Original language | Spanish |
| Pages (from-to) | 1009-1017 |
| Number of pages | 9 |
| Journal | Revista de Investigaciones Veterinarias del Peru |
| Volume | 29 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2018 |
Bibliographical note
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